Studies on relationships between air pollutants and allergenicity of Humulus Scandens pollen collected from different areas of Shanghai
Studies on relationships between air pollutants and allergenicity of Humulus Scandens pollen collected from different areas of Shanghai
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上海不同地区空气污染物与葎草花粉致敏性关系研究
DOI：
10.1016/j.jes.2020.03.037
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发表时间：
2020
影响因子：
6.9
通讯作者：
Wang Qingyue
中科院分区：
文献类型：
--
作者：
Lu Senlin;Yao Chuanhe;Zhou Shumin;Lin Yichun;Zhang Luying;Zeng Junyang;Rao Lanfang;Zhang Wei;Dai Yafeng;Li Hong;Wang Weiqian;Wang Qingyue
花粉污染和过敏已成为中国的突出问题。然而，关于花粉病的研究报道很少。作为大气中的过敏原，过敏性葎草花粉是从上海吴淞（WS）、嘉定（JD）、徐家汇（XJH）和松江（SJ）四个区采集的。葎草花粉季节期间，还记录了四个采样点附近的 SO2、NO2、O3、PM10 和 PM2.5（空气动态直径分别小于 10 和 2.5 µm 的颗粒物）的质量浓度。通过使用大鼠模型和酶联免疫吸附测定(ELISA)来评估葎草花粉的变应原性。过敏性和空气污染物之间的关系是相关的。我们的结果表明，从四个地区收集的花粉的生物活力没有表现出显着差异。 ELISA和斑点印迹结果进一步表明致敏大鼠的血清表现出比对照组高得多的免疫反应性反应。蛋白质印迹表明，葎草花粉的 15 KD（1KD = 1000 道尔顿）蛋白质导致了过敏反应。不同样品的葎草花粉蛋白致敏强度依次为：WS>JD>XJ>SJ。葎草花粉的致敏性与PM10(R=-0.99)/PM2.5(R=-0.73)呈负相关，与O3呈正相关(R=0.92)。这些数据清楚地表明PM10和PM2.5可以增强葎草花粉蛋白的释放，而O3可以加剧葎草花粉的致敏性。
Pollen pollution and allergy are becoming prominent issues in China. However, few studies on pollinosis have been reported. As an allergen in the atmosphere, allergenicHumulus scandenspollen was collected from four districts of Shanghai, including Wusong (WS), Jiading (JD), Xujiahui (XJH) and Songjiang (SJ). The mass concentrations of SO2, NO2, O3, PM10,and PM2.5(particulate matter with air dynamic diameter less than 10 and 2.5 µm, respectively) near the four sampling sites were also recorded duringHumulus scandenspollen season. The allergenicity of theHumulus scandenspollen was assessed by using of a rat model and enzyme linked immunosorbent assay (ELISA). Relationships between the allergenicity and air pollutants were correlated. Our results demonstrated that the biological viability of the pollens collected from the four districts exhibited no significant differences. ELISA and dot blotting results further demonstrated that the serum of sensitized rats exhibited much higher immune-reactive response than that of control groups. Western blotting showed that the 15 KD (1KD = 1000 dalton) proteins ofHumuluspollen led to the allergic response. The allergenic intensity ofHumuluspollen protein from different samples followed the pattern: WS > JD > XJ > SJ. There was a negative relationship between the allergenicity ofHumuluspollens and PM10(R= -0.99) / PM2.5(R= -0.73), and a positive relationship with O3(R= 0.92). These data clearly showed that PM10and PM2.5could enhanceHumuluspollen protein release, and O3could aggravate the allergenicity of theHumuluspollen.