Matrix-assisted laser desorption ionization mass spectrometry: a new tool for probing interactions between proteins and metal surfaces. Use in dental implantology.
Matrix-assisted laser desorption ionization mass spectrometry: a new tool for probing interactions between proteins and metal surfaces. Use in dental implantology.
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基质辅助激光解吸电离质谱：探测蛋白质和金属表面之间相互作用的新工具。
DOI：
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发表时间：
1999
影响因子：
2.9
通讯作者：
A. van Dorsselaer
中科院分区：
文献类型：
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作者：
E. Leize;E. Leize;M. Leize;J. Voegel;A. van Dorsselaer
人工钛牙根在骨中的固定被认为是通过手术腔中存在的蛋白质在钛表面上的快速吸附而开始的。这种吸附的研究应该能够预测新的种植体表面处理的骨整合能力。在这里，我们描述了一种新的方法，基于基质辅助激光解吸电离质谱（MALDI-MS），用于定量蛋白质吸附在钛表面完全相同的设计用于种植。该方法的关键步骤是一种新的MALDI-MS样品制备，允许吸附的蛋白质从表面去除并均匀分散在基质晶体中。研究了模型蛋白质（溶菌酶）在两个钛表面（抛光和喷砂）上的吸附，以评估该方法。MALDI-MS的绝对强度与吸附的溶菌酶量呈线性关系。将钛表面在不同浓度的溶菌酶溶液中浸渍不同时间后，通过MALDI-MS测量吸附的溶菌酶的最大量，并显示对应于溶菌酶单层，这与文献中描述的结果一致。
The fixation in the bone of an artificial titanium tooth root is believed to be initiated by the rapid adsorption of the proteins present in the surgical cavity on the titanium surface. The study of this adsorption should make it possible to predict the osseointegration capacities of new implant surface treatments. We describe here a new method, based on matrix-assisted laser desorption ionization-mass spectrometry (MALDI-MS), for quantifying proteins adsorbed on titanium surfaces fully identical to these designed for implantology. The key step of this method is a new MALDI-MS sample preparation allowing the adsorbed proteins to be removed from the surface and to be homogeneously dispersed in the matrix crystals. The adsorption of a model protein (lysozyme) on two titanium surfaces (polished and sandblasted) was studied in order to evaluate the method. The absolute MALDI-MS intensity was shown to vary linearly with the amount of adsorbed lysozyme. After dipping the titanium surfaces for different times in lysozyme solutions at different concentrations, the maximum amount of adsorbed lysozyme was measured by MALDI-MS and was shown to correspond to a lysozyme monolayer, which is consistent with results described in the literature.