A solid-in-oil nanodispersion for transcutaneous protein delivery.
A solid-in-oil nanodispersion for transcutaneous protein delivery.
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DOI：
10.1016/j.jconrel.2008.07.015
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发表时间：
2008-10
期刊：
影响因子：
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通讯作者：
Y. Tahara;Shota Honda;N. Kamiya;Hongyu Piao;Akihiko Hirata;E. Hayakawa;T. Fujii;M. Goto
中科院分区：
文献类型：
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作者：
Y. Tahara;Shota Honda;N. Kamiya;Hongyu Piao;Akihiko Hirata;E. Hayakawa;T. Fujii;M. Goto
亲水性大分子药物的经皮给药备受关注，但仍是一个具有挑战性的策略。在本研究中，我们证明了油包固体（S/O）纳米分散体，亲水性药物的油基纳米分散体，有效地增强了蛋白质渗透到皮肤中。所有不同的模型蛋白质，FITC标记的胰岛素（MW约为1000），6 kDa）、增强型绿色荧光蛋白（EGFP，MW ca. 27 kDa）和辣根过氧化物酶（HRP，MW约为2.5 kDa）。40 kDa），通过形成S/O纳米分散体渗透通过尤卡坦小型猪皮肤的角质层。穿透的EGFP和HRP分别表现出绿色荧光和催化活性，表明这些蛋白质可以以功能形式渗透到皮肤中。结果表明S/O纳米分散体作为经皮蛋白质递送的新型载体的潜在效用。
Transcutaneous delivery attracts much attention but remains a challenging strategy for hydrophilic macromolecular drug administration. In the present study, we demonstrated that a solid-in-oil (S/O) nanodispersion, an oil-based nanodispersion of hydrophilic drugs, effectively enhanced the permeation of proteins into the skin. All of the different model proteins, FITC-labeled insulin (MW ca. 6 kDa), enhanced green fluorescent protein (EGFP, MW ca. 27 kDa) and horseradish peroxidase (HRP, MW ca. 40 kDa), permeated through the stratum corneum of Yucatan micropig skin in vitro by forming a S/O nanodispersion. The penetrated EGFP and HRP exhibited green fluorescence and catalytic activity, respectively, suggesting that these proteins can permeate into the skin in a functional form. The results indicated the potential utility of the S/O nanodispersion as a novel vehicle for transcutaneous protein delivery.