Genotype differentiating RT-PCR and sandwich ELISA: Handy tools in epidemiological investigation of foot and mouth disease
Genotype differentiating RT-PCR and sandwich ELISA: Handy tools in epidemiological investigation of foot and mouth disease
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DOI：
10.1016/j.jviromet.2007.02.008
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发表时间：
2007-07-01
影响因子：
3.1
通讯作者：
Rasool, Thaha Jamal
中科院分区：
文献类型：
--
作者：
Mohapatra, Jajati Keshari;Subramaniam, Saravanan;Rasool, Thaha Jamal
多年的分子流行病学监测显示，在印度存在两种抗原性不同的A型口蹄疫病毒基因型的共同传播。基因型区分RT-PCR和夹心ELISA被开发为用于检测和区分基因型VI和VII的基于ID区域的同源性的快速、成本有效和用户友好的替代方案。以ID区为靶点的RT-PCR方法比夹心ELISA方法更敏感、可靠。这些检测方法有望成为该国口蹄疫流行病学调查的可靠工具。(C)2007年Elsevier B. V.。All rights reserved.
Years of molecular epidemiological surveillance has revealed co-circulation of two antigenically divergent genotypes of foot and mouth disease virus serotype A in India. Genotype differentiating RT-PCR and sandwich ELISA were developed as fast, cost-effective and user-friendly alternatives to I D region based phylogeny for detection and differentiation of genotype VI and VII. The RT-PCR assay targeting ID region was found to be more sensitive and authentic in distinguishing genotypes than sandwich ELISA. These assays promise to be reliable tools in the epidemiological investigation of foot and mouth disease in the country. (C) 2007 Elsevier B.V.. All rights reserved.