The global virome: not as big as we thought?
The global virome: not as big as we thought?
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DOI：
10.1016/j.coviro.2013.07.004
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发表时间：
2013-10-01
影响因子：
5.9
通讯作者：
Sullivan, Matthew B.
中科院分区：
文献类型：
--
作者：
Ignacio-Espinoza, J. Cesar;Solonenko, Sergei A.;Sullivan, Matthew B.
病毒可能会感染所有生物体，在复杂的生物体网络中充当遗传载体的程度尚不清楚。环境病毒学家透露，这些丰富的纳米级实体是全球参与者，在所研究的每个生态系统中都扮演着关键角色。奇怪的是，新基因主导着病毒基因组和元基因组，这导致了文献估计，根据14个分枝杆菌噬菌体基因组推断，病毒是地球上最大的未被探索的遗传物质储存库，这表明仍有20亿个噬菌体编码的ORF有待发现。在这里，我们研究了自这一挑衅性断言以来十年来可用的(Meta)基因组数据，并使用“蛋白质簇”来评估采样技术是否进步到了能够对自然界中的病毒多样性进行“全部”采样的地步。
Viruses likely infect all organisms, serving to unknown extent as genetic vectors in complex networks of organisms. Environmental virologists have revealed that these abundant nanoscale entities are global players with critical roles in every ecosystem investigated. Curiously, novel genes dominate viral genomes and metagenomes, which has led to the suggestion that viruses represent the largest reservoir of unexplored genetic material on Earth with literature estimates, extrapolating from 14 mycobacteriophage genomes, suggesting that two billion phage-encoded ORFs remain to be discovered. Here we examine (meta)genomic data available in the decade since this provocative assertion, and use 'protein clusters' to evaluate whether sampling technologies have advanced to the point that we may be able to sample 'all' of viral diversity in nature.