Cooperative Phagocytes Resident Microglia and Bone Marrow Immigrants Remove Dead Photoreceptors in Retinal Lesions
Cooperative Phagocytes Resident Microglia and Bone Marrow Immigrants Remove Dead Photoreceptors in Retinal Lesions
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DOI：
10.2353/ajpath.2009.090023
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发表时间：
2009-06-01
影响因子：
6
通讯作者：
Reme, Charlotte E.
中科院分区：
文献类型：
--
作者：
Joly, Sandrine;Francke, Mike;Reme, Charlotte E.
吞噬作用对于视网膜损伤后光感受器碎片的清除是必不可少的。我们使用了两种小鼠模型，即注射绿色荧光蛋白标记的骨髓细胞或绿色荧光蛋白标记的小胶质细胞的小鼠，来研究急性蓝光诱导的视网膜病变后吞噬细胞的起源和激活模式。我们发现，损伤后，血液传播的巨噬细胞通过视神经和睫状体进入眼睛，并很快迁移到受伤的视网膜区域。常驻小胶质细胞也在整个视网膜中迅速激活，并仅在受伤区域采用巨噬细胞特征。血液和小胶质细胞衍生的巨噬细胞都参与死亡光感受器的吞噬作用。未观察到血-视网膜屏障的明显破坏。Cc14、Cc112、Tgfb1、Csf1和Tnf在野生型小鼠的离体视网膜和眼杯中差异表达。充满碎片的巨噬细胞似乎离开视网膜进入全身循环，这表明它们有可能成为抗原呈递细胞。这些实验提供了证据表明，本地和移民巨噬细胞清除凋亡的光感受器和细胞碎片在受损的视网膜。(Am J Pathol 2009，174：2310 - 2323; DOI：10.2353/ajpath.2009.090023）
Phagocytosis is essential for the removal of photoreceptor debris following retinal injury. We used two mouse models, mice injected with green fluorescent protein-labeled bone marrow cells or green fluorescent protein-labeled microglia, to study the origin and activation patterns of phagocytic cells after acute blue light-induced retinal lesions. We show that following injury, blood-borne macrophages enter the eye via the optic nerve and ciliary body and soon migrate into the injured retinal area. Resident microglia are also activated rapidly throughout the entire retina and adopt macrophage characteristics only in the injured region. Both blood-borne and microglia-derived macrophages; were involved in the phagocytosis of dead photoreceptors. No obvious breakdown of the blood-retinal barrier was observed. Cc14, Cc112, Tgfb1, Csf1, and Tnf were differentially expressed in both the isolated retina and the eyecup of wild-type mice. Debris-laden macrophages appeared to leave die retina into the general circulation, suggesting their potential to become antigen-presenting cells. These experiments provide evidence that both local and immigrant macrophages remove apoptotic photoreceptors and cell debris in the injured retina. (Am J Pathol 2009, 174:2310-2323; DOI: 10.2353/ajpath.2009.090023)