1H NMR studies of nickel(II) complexes bound to oligonucleotides: a novel technique for distinguishing the binding locations of metal complexes in DNA.
1H NMR studies of nickel(II) complexes bound to oligonucleotides: a novel technique for distinguishing the binding locations of metal complexes in DNA.
复制标题
与寡核苷酸结合的镍 (II) 配合物的 1H NMR 研究：一种区分 DNA 中金属配合物结合位置的新技术。
DOI：
10.1021/ic0348291
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发表时间：
2003
期刊：
影响因子：
--
通讯作者：
Barton,JacquelineK
中科院分区：
文献类型：
--
作者：
Bhattacharya,PratipK;Lawson,HollyJ;Barton,JacquelineK
The selective paramagnetic relaxation of oligonucleotide proton resonances of d(GTCGAC)2and d(GTGCAC)2by Ni(phen)2(L)2+where L = dipyridophenazine (dppz), dipyrido[3,2-d:2‘,3‘-f]quinoxaline (dpq), and phenanthrenequinone (phi) has been examined to obtain structural insight into the noncovalent binding of these metal complexes to DNA. In the oligonucleotide d(GTCGAC)2, preferential broadening of the G1H8, G4H8, T2H6, and C3H6 proton resonances was observed with Ni(phen)2(dppz)2+, Ni(phen)2(dpq)2+, and Ni(phen)2(phi)2+. In the case of the sequence d(GTGCAC)2, where the central two bases are juxtaposed from the previous one, preferential broadening was observed instead for the A5H2 proton resonance. Thus, a subtle change in the sequence of the oligonucleotide can cause significant change in the binding location of the metal complex in the oligonucleotide. Owing to comparable changes for all metal complexes and sequences in broadening of the thymine methyl proton resonances, we attribute the switch in preferential broadening to a change in site location within the oligomer rather than to an alteration of groove location. Therefore, even for DNA-binding complexes of low sequence-specificity, distinct variations in binding as a function of sequence are apparent.
