Involvement of the nerve growth factor-inducible large external glycoprotein (NILE) in neurite fasciculation in primary cultures of rat brain.
Involvement of the nerve growth factor-inducible large external glycoprotein (NILE) in neurite fasciculation in primary cultures of rat brain.
复制标题
神经生长因子诱导的大外部糖蛋白（NILE）参与大鼠脑原代培养物的神经突束颤。
DOI：
10.1073/pnas.82.4.1276
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发表时间：
1985
影响因子：
11.1
通讯作者：
Beasley,L
中科院分区：
文献类型：
--
作者：
Stallcup,WB;Beasley,L
神经生长因子诱导的大外部糖蛋白（NILE）仅存在于神经元细胞和雪旺细胞的表面。由于NILE似乎是集中在神经突，我们已经调查了其可能的作用，在原代培养的大鼠脑神经突的发展。将胚胎14天（E14）的全脑培养物和出生后5天（P5）的小脑培养物在抗NILE抗体的Fab'片段存在下生长，试图干扰神经突发育的正常模式。为了进行比较，用两种识别神经元细胞表面分子的其他试剂处理培养物：破伤风毒素，其结合GD 1b和GT 1神经节苷脂，以及抗神经细胞粘附分子（N-CAM）的抗体的Fab'片段。在所使用的条件下，没有外源性试剂影响神经突生长，但观察到对神经突成束的具体影响。抗NILE抑制E14全脑培养物中的肌束震颤，但对P5小脑培养物中的肌束震颤没有影响。相反，抗-N-CAM抑制在P5小脑，其中包含成人形式的N-CAM的文化，但在E14全脑，其中包含N-CAM的胚胎形式的文化中的成束的影响很小。破伤风毒素对两种培养体系中的肌束震颤均无影响。我们的研究结果表明，NILE介导的神经突-神经突的相互作用是强于N-CAM（胚胎）介导的相互作用在E14脑文化，而N-CAM（成人）介导的相互作用是强于NILE介导的相互作用在P5小脑文化。
The nerve growth factor-inducible large external glycoprotein (NILE) has been found only on the surface of neuronal cells and Schwann cells. Since NILE seems to be concentrated on neurites, we have investigated its possible role in the development of neurites in primary cultures of rat brain. Cultures of embryonic day 14 (E14) whole brain and cultures of postnatal day 5 (P5) cerebellum were grown in the presence of Fab' fragments of antibody against NILE in an attempt to perturb the normal pattern of neurite development. For comparison, cultures were treated with two other reagents that recognize neuronal cell surface molecules: tetanus toxin, which binds to the GD1b and GT1 gangliosides, and Fab' fragments of antibody against neural cell adhesion molecule (N-CAM). Under the conditions used, none of the exogenous reagents affected neurite outgrowth, but specific effects on neurite fasciculation were observed. Anti-NILE inhibited fasciculation in cultures of E14 whole brain but had no effect on fasciculation in cultures of P5 cerebellum. Conversely, anti-N-CAM inhibited fasciculation in cultures of P5 cerebellum, which contain the adult form of N-CAM, but had little effect on fasciculation in cultures of E14 whole brain, which contain the embryonic form of N-CAM. Tetanus toxin had no effect on fasciculation in either culture system. Our results imply that NILE-mediated neurite-neurite interactions are stronger than N-CAM (embryonic)-mediated interactions in the E14 brain cultures, whereas N-CAM (adult)-mediated interactions are stronger than NILE-mediated interactions in the P5 cerebellar cultures.