Sensitive and Quantitative Determination of Cardiac Troponin I Based on Silica-Encapsulated CdSe/ZnS Quantum Dots and a Fluorescence Lateral Flow Immunoassay
Sensitive and Quantitative Determination of Cardiac Troponin I Based on Silica-Encapsulated CdSe/ZnS Quantum Dots and a Fluorescence Lateral Flow Immunoassay
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DOI：
10.1080/00032719.2020.1719125
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发表时间：
2020-03
影响因子：
2
通讯作者：
Min Wu;Xian Zhang;Ruili Wu;Guochun Wang;Jinjie Li;Yujuan Chai;Huaibin Shen;L. Li
中科院分区：
文献类型：
--
作者：
Min Wu;Xian Zhang;Ruili Wu;Guochun Wang;Jinjie Li;Yujuan Chai;Huaibin Shen;L. Li
摘要 心肌肌钙蛋白I通常用作心肌梗死或急性心肌梗死的生物标志物。及时、快速的心肌肌钙蛋白I诊断方法具有重要意义。在本文中，报道了一种新型基于 CdSe/ZnS 量子点 (QD) 的荧光侧流免疫分析 (QD-LFIA)，用于快速定量检测人血清中的心肌肌钙蛋白 I。水溶性二氧化硅封装的量子点(QDs@SiO2)作为一种新型荧光探针，首次使用经济、便携的定量仪器来检测心肌肌钙蛋白I抗原。采用优化的临床样本免疫分析流程，10分钟即可测定心肌肌钙蛋白I，灵敏度高（低至5.6×10−3 ng/mL），线性范围宽（0.8-200ng/mL），精密度好（变异系数小于10%）。这种新型 QD-LFIA 与标准直接化学发光检测的比较表明，前一种平台快速、低成本且灵敏。其可进一步应用于临床上心肌肌钙蛋白I水平的定量测定和监测。
Abstract Cardiac troponin I is generally used as a biomarker of myocardial infarction or acute myocardial infarction. A timely and rapid diagnosis method for cardiac troponin I is of great importance. In this paper, a novel CdSe/ZnS quantum dot (QD) based fluorescence lateral flow immunoassay (QD-LFIA) is reported for the rapid and quantitative detection of cardiac troponin I in human serum. The water-soluble silica-encapsulated QDs (QDs@SiO2) serve as a new fluorescent probe and an economical, portable, quantitative instrument was used for the first time to detect cardiac troponin I antigen. By using the optimized immunoassay procedure with clinical samples, cardiac troponin I was determined in 10 min with high sensitivity (as low as 5.6 × 10−3 ng/mL), broad linear range (0.8–200 ng/mL), and good precision (the coefficient of variation was less than 10%). The comparison of this new QD-LFIA and a standard direct chemiluminescence assay demonstrated that the former platform was rapid, low-cost, and sensitive. It may be further applied to the quantitative determination and monitoring of cardiac troponin I levels in clinical use.