A role for nitrergic system in the antidepressant-like effects of chronic lithium treatment in the mouse forced swimming test
A role for nitrergic system in the antidepressant-like effects of chronic lithium treatment in the mouse forced swimming test
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DOI：
10.1016/j.bbr.2008.12.032
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发表时间：
2009-06-08
影响因子：
2.7
通讯作者：
Dehpour, Ahmad Reza
中科院分区：
文献类型：
--
作者：
Ghasemi, Mehdi;Sadeghipour, Hamed;Dehpour, Ahmad Reza
在本研究中，我们评估了一氧化氮（NO）系统参与慢性锂给药在小鼠强迫游泳试验（FST）的抗抑郁样作用。给予氯化锂（300 mg/L在饮用水中21 d）对小鼠在FST中的不动性没有影响，而600 mg/L锂与对照动物相比引起不动时间显著减少（P < 0.001）。将NO合成酶抑制剂N-G-硝基-L-精氨酸甲酯（L-NAME，10 mg/kg，每天一次，持续一周，i. p.）对对照或锂（300 mg/L）处理的小鼠的不动时间没有显著影响，而急性给予无效剂量的L-NAME（30 mg/kg，i. p.）在FST中，锂（300 mg/L）处理的动物的不动时间显著减少（P < 0.01）。此外，长期施用低剂量的NO前体L-精氨酸（200 mg/kg，每天持续一周，i. p.）在FST中阻止了锂处理（600 mg/L）的抗抑郁样作用（P < 0.001）。用L-精氨酸（200 mg/kg，i. p.）增加锂（600 mg/L）处理小鼠在FST中的不动时间（P < 0.05）。慢性锂处理（600 mg/L而非300 mg/L）导致小鼠血清NOX水平与对照组相比显著降低（P < 0.05）。我们的数据表明，NO系统可能参与了慢性锂治疗FST的抗抑郁样作用。(C)2009 Elsevier B. V.保留所有权利。
In the present study we evaluated the involvement of nitric oxide (NO) system in the antidepressant-like effects of chronic lithium administration in the mouse forced swimming test (FST). Administration of lithium chloride (300 mg/L in drinking water for 21 days) had no effect on the immobility of mice in the FST, whereas 600 mg/L lithium caused a significant (P < 0.001) decrease in the immobility time compared with control animals. Administration of the NO synthase inhibitor N-G-nitro-L-arginine methyl ester (L-NAME, 10 mg/kg, daily for a week, i.p.) had no significant effect on the immobility time of either control or lithium (300 mg/L)-treated mice, whereas acute administration of non-effective dose of L-NAME (30 mg/kg, i.p.) caused a robust decrease (P < 0.01) in the immobility time of lithium (300 mg/L)-treated animals in the FST. Moreover, chronic administration of low dose of the NO precursor L-arginine (200 mg/kg, daily for a week, i.p.) prevented (P < 0.001) the antidepressant-like effects of lithium treatment (600 mg/L) in the FST. Acute treatment with L-arginine (200 mg/kg, i.p.) increased (P < 0.05) the immobility time of lithium (600 mg/L)-treated mice in the FST. Chronic lithium treatment (600 mg/L but not 300 mg/L) caused a significant (P < 0.05) decrease in the serum NOX levels in mice compared with controls. Our data suggested that the NO system could be involved in the antidepressant-like effect of chronic lithium treatment in the FST. (C) 2009 Elsevier B.V. All rights reserved.