Evaluation of a new experimental kit for the extraction of DNA from bones and teeth using a non-powder method
Evaluation of a new experimental kit for the extraction of DNA from bones and teeth using a non-powder method
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DOI：
10.1016/j.legalmed.2009.12.004
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发表时间：
2010-03-01
期刊：
影响因子：
1.5
通讯作者：
Fukushima, Hirofumi
中科院分区：
文献类型：
--
作者：
Kitayama, Tetsushi;Ogawa, Yoshinori;Fukushima, Hirofumi
最近开发了一种实验性DNA提取试剂盒（新试剂盒），用于从降解的骨骼遗骸中提取DNA，而无需对样品进行粉末处理。我们使用实时定量PCR和多重STR分析将新试剂盒与传统的苯酚/氯仿方法进行了比较。与传统的苯酚/氯仿方法相比，新的试剂盒从紧凑的骨碎片中产生了大量的DNA。使用新试剂盒，我们能够分别从75%（3 / 4）和60%（3 / 5）的未粉状牙齿和骨骼样本中提取足够的DNA进行STR分析。我们能够使用新试剂盒提取的DNA对剩余样品进行mini-STR分析。此外，我们成功地对每个样本进行了线粒体DNA测序。新试剂盒简化了DNA提取过程，因为它不需要粉末样品。减少DNA提取的程序步骤将有利于控制实验室中的DNA污染。我们的结果表明，新的试剂盒可用于简单，同时从多个样品中提取DNA。2010爱思唯尔爱尔兰有限公司版权所有。
An experimental DNA extraction kit (new kit) was recently developed to extract DNA from degraded skeletal remains without the need for powdering the samples. We compared the utility of the new kit with the conventional phenol/chloroform method using real-time quantitative PCR and multiplex STR analysis. The new kit yielded large amounts of DNA from a compact bone fragment compared with the conventional phenol/chloroform method. We were able to extract sufficient DNA for STR analysis from 75% (3 of 4) and 60% (3 of 5) of the un-powdered tooth and bone samples, respectively, using the new kit. We were able to perform mini-STR analysis of the remaining samples using DNA extracted with the new kit. Furthermore, we successfully performed mitochondrial DNA sequencing of every sample. The new kit simplifies the DNA extraction procedure as it does not require powdering samples. Decreasing the number of procedural steps in DNA extraction will be beneficial in controlling DNA contamination in laboratories. Our results suggest that the new kit may be used for the simple, simultaneous extraction of DNA from multiple samples. (C) 2010 Elsevier Ireland Ltd. All rights reserved.