Mucin expression in endoscopic ultrasound-guided fine-needle aspiration specimens is a useful prognostic factor in pancreatic ductal adenocarcinoma.
Mucin expression in endoscopic ultrasound-guided fine-needle aspiration specimens is a useful prognostic factor in pancreatic ductal adenocarcinoma.
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DOI：
10.1097/mpa.0000000000000362
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发表时间：
2015-07
期刊：
影响因子：
2.9
通讯作者：
Yonezawa S
中科院分区：
文献类型：
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作者：
Higashi M;Yokoyama S;Yamamoto T;Goto Y;Kitazono I;Hiraki T;Taguchi H;Hashimoto S;Fukukura Y;Koriyama C;Mataki Y;Maemura K;Shinchi H;Jain M;Batra SK;Yonezawa S
本研究的目的是进一步研究粘蛋白表达谱作为PDAC预后因素的效用。采用免疫组化（IHC）方法检测114例PDAC患者超声引导下细针穿刺（EUS-FNA）标本中Mucin （MUC）的表达。将各粘蛋白的表达率与临床病理特征进行比较。mucins在癌灶中的表达率MUC1, 87.7%；MUC2, 0.8%;MUC4, 93.0%;MUC5AC, 78.9%;MUC6, 24.6%;MUC16占67.5%。MUC1、MUC4阳性，MUC2阴性。MUC5AC表达的晚期PDAC患者预后明显优于MUC5AC阴性患者（P=0.002）。随着临床分期的增加，MUC6总表达量降低（P趋势=0.001），MUC16细胞质表达量升高（P趋势=0.02）。MUC16细胞质表达的患者预后明显差于未表达的患者。多因素生存分析显示，在调整其他预后因素的影响后，MUC16细胞质表达是预后不良的重要独立预测因子（P=0.002）。EUS-FNA标本中的粘蛋白表达谱具有出色的诊断功能，是PDAC患者预后的有用预测因子。
The aim of this study was to further examine the utility of mucin expression profiles as prognostic factors in PDAC. Mucin (MUC) expression was examined by immunohistochemistry (IHC) analysis in endoscopic ultrasound-guided fine-needle aspiration (EUS-FNA) specimens obtained from 114 patients with PDAC. The rate of expression of each mucin was compared with clinicopathologic features. The expression rates of mucins in cancer lesions were MUC1, 87.7%; MUC2, 0.8%; MUC4, 93.0%; MUC5AC, 78.9%; MUC6, 24.6%; and MUC16, 67.5%. MUC1 and MUC4 were positive and MUC2 was negative in most PDACs. Patients with advanced stage of PDAC with MUC5AC expression had a significantly better outcome than those who were MUC5AC-negative (P=0.002).With increasing clinical stage, total MUC6 expression decreased (P for trend=0.001) and MUC16 cytoplasmic expression increased (P for trend=0.02). The prognosis of patients with MUC16 cytoplasmic expression was significantly poorer than those without this expression. Multivariate survival analysis revealed that MUC16 cytoplasmic expression was a significant independent predictor of a poor prognosis after adjusting for the effects of other prognostic factors (P=0.002). Mucin expression profiles in EUS-FNA specimens have excellent diagnostic utility and are useful predictors of outcome in patients with PDAC.