Efficient elimination and detection of phenolic compounds in juice using laccase mimicking nanozymes
Efficient elimination and detection of phenolic compounds in juice using laccase mimicking nanozymes
复制标题
使用漆酶模拟纳米酶有效消除和检测果汁中的酚类化合物
DOI：
10.1016/j.cjche.2020.04.012
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发表时间：
2021-01-01
影响因子：
3.8
通讯作者：
Li, Yongxin
中科院分区：
文献类型：
--
作者：
Huang, Hui;Lei, Lulu;Li, Yongxin
果汁中残留的酚类物质会引起浑浊，影响其感官质量。漆酶用于去除果汁中的酚类化合物。为了克服天然漆酶不稳定和成本高的缺点，本工作制备了一种基于铜离子和单磷酸腺苷的漆酶模拟酶（AMP-Cu纳米酶）。在相同质量浓度（1 mg.ml(-1)）下，纳米酶的催化活性约为漆酶的15倍。 AMP-Cu 纳米酶比漆酶具有更高的 V-max 和更低的 K-m。漆酶模拟酶在30-90℃和pH N > 6的条件下具有良好的稳定性。在高盐浓度和9天的储存时间下也保持较高的催化活性。此外，AMP-Cu纳米酶在连续六个反应循环后仍保持约80%的初始催化活性。 AMP-Cu纳米酶检测酚类化合物的线性范围为0.1~100 mu mol.L-1,检出限为0.033 mu mol.L-1。不同反应时间下AMP-Cu纳米酶的苯酚去除率均远高于漆酶。当反应进行5 h时，AMP-Cu纳米酶对果汁的苯酚去除率约为65%。 AMP-Cu纳米酶对不同果汁中酚类化合物的高效去除表明其在食品果汁行业具有良好的应用前景。 (C)2020 中国化学工业工程学会、化学工业出版社有限公司 版权所有。
Residual phenols in the juice can cause turbidity and affect its sensory quality. Laccase is used to remove phenolic compounds fromfruit juices. In order to overcomethe shortcomings of natural laccase instability and high cost, in this work, we prepared a laccase mimic enzyme based on copper ion and adenosine monophosphate (AMP-Cu nanozymes). At the same mass concentration (1 mg.ml(-1)), the catalytic activity of the nanozyme is about 15 times that of laccase. The AMP-Cu nanozymes had a higher V-max and a lower K-m than laccase. The laccase mimic enzyme had a good stability under the condition of 30-90 degrees C and pH N > 6. It also maintained high catalytic activity at high salt concentrations and 9 days storage time. Furthermore, the AMP-Cu nanozymes maintained an initial catalytic activity of about 80% after six consecutive cycles of reaction. The linear range of detection of phenolic compounds by AMP-Cu nanozymes was 0.1-100 mu mol.L-1 with a detection limit of 0.033 mu mol.L-1. The phenol removal rate of AMP-Cu nanozymes was much higher than that of laccase under different reaction times. When the reactionwas performed for 5 h, the phenol removal rate of the fruit juice byAMP-Cu nanozymes was about 65%. The efficient removal of phenolic compounds from different juices by AMP-Cu nanozymes indicates that they have good application prospect in the food juice industry. (C) 2020 The Chemical Industry and Engineering Society of China, and Chemical Industry Press Co., Ltd. All rights reserved.