Polyamine oxidase 2 is involved in regulating excess spermidine contents during seed germination and early seedling development in Arabidopsis thaliana
Polyamine oxidase 2 is involved in regulating excess spermidine contents during seed germination and early seedling development in Arabidopsis thaliana
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DOI：
10.1016/j.bbrc.2019.07.022
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发表时间：
2019-09-03
影响因子：
3.1
通讯作者：
Teshima, Yuuta
中科院分区：
文献类型：
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作者：
Takahashi, Yoshihiro;Uemura, Tatsuaki;Teshima, Yuuta
为了解植物多胺氧化酶（PAO）的生理功能，以拟南芥（Arabidopsis thaliana）中T-DNA插入的PAO基因为材料，分析了外源多胺对种子萌发和幼苗早期发育的影响。精胺引起幼苗生长抑制，但不影响发芽在所有行，包括野生型Col-0。然而，AtPAO 2敲除突变体，-pao 2，不能发芽下过量亚精胺（Spd）的条件。根的生长速率发芽后pao 2幼苗也强烈抑制亚精胺处理相比，野生型植物。AtPAO 2在其C-末端具有保守的过氧化物酶体靶向信号序列。我们在a-pao 2背景下制备了两种类型的AtPAO 2表达植物。在-pao 2/PAO 2植物中引入含有AtPAO 2的完整编码序列的5.8-kbp基因组片段，而在-pao 2/PAO 2（Δ C）植物中引入缺乏过氧化物酶体靶向信号的相同片段。在-pao 2中观察到的Spd敏感表型在两个转基因互补系中完全恢复。因此，AtPAO 2似乎参与过量的Spd catalysts在种子萌发和早期幼苗发育的亚细胞定位无关。(C)2019爱思唯尔公司All rights reserved.
To understand the physiological functions of polyamine oxidases (PAOs) in plants, we analyzed the effects of exogenous polyamines during seed germination and early seedling development, using Arabidopsis thaliana lines independently harboring T-DNA insertions in each PAO gene. Spermine caused seedling growth inhibition but did not affect the germination in all lines including wild-type Col-0. However, an AtPAO2 knockout mutant, -pao2, could not germinate under excess spermidine (Spd) conditions. The root growth rates of post-germination -pao2 seedlings were also strongly inhibited by the Spd treatment compared with the wild-type plants. AtPAO2 has a conserved peroxisome-targeting signal sequence at its C-terminus. We prepared two types of AtPAO2 expression plants in a -pao2 background. In -pao2/PAO2 plants a 5.8-kbp genomic fragment containing the complete coding sequence of AtPAO2 was introduced, while in -pao2/PAO2(Delta C) plants the same fragment lacking the peroxisome-targeting signal was introduced. The Spd-sensitive phenotypes observed in -pao2 were completely recovered in both of the transgenic complementation lines. Thus, AtPAO2 appears to be involved in excess Spd catabolism during seed germination and early seedling development irrespective of subcellular localization. (C) 2019 Elsevier Inc. All rights reserved.