CLONING AND SEQUENCING OF THE SACA GENE - CHARACTERIZATION OF A SUCRASE FROM ZYMOMONAS-MOBILIS
CLONING AND SEQUENCING OF THE SACA GENE - CHARACTERIZATION OF A SUCRASE FROM ZYMOMONAS-MOBILIS
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DOI：
10.1128/jb.172.12.6727-6735.1990
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发表时间：
1990-12-01
影响因子：
3.2
通讯作者：
BARATTI, J
中科院分区：
文献类型：
--
作者：
GUNASEKARAN, P;KARUNAKARAN, T;BARATTI, J
运动发酵菌（Zymomoas mobilis）基因（sacA）编码一种具有蔗糖酶活性的蛋白质，已在大肠杆菌中克隆并测定了其核苷酸序列。在该基因上游区域发现了与大肠杆菌同源的潜在核糖体结合位点和启动子序列。coli和Z. mobilis共有序列。E.大肠杆菌细胞，含有sacA基因，显示蔗糖水解活性。然而，未检测到转果糖基化活性（交换反应或莱万形成）。这种蔗糖酶活性与用纯化的来自Z的胞外蛋白B46观察到的不同。mobilis。这两种蛋白质的电泳迁移率和分子量不同，且在免疫学上没有相似之处。因此，sacA（一种分子量为58.4kDa的多肽）的产物是一种新的Z. mobilis。从sacA的核苷酸序列推导的氨基酸序列，与枯草芽孢杆菌，鼠伤寒沙门氏菌和溶藻弧菌的蔗糖酶显示出很强的同源性。
The Zymomoas mobilis gene (sacA) encoding a protein with sucrase activity has been cloned in Escherichia coli and its nucleotide sequence has been determined. Potential ribosome-binding site and promoter sequences were identified in the region upstream of the gene which were homologous to E. coli and Z. mobilis consensus sequences. Extracts from E. coli cells, containing the sacA gene, displayed a sucrose-hydrolyzing activity. However, no transfructosylation activity (exchange reaction or levan formation) could be detected. This sucrase activity was different from that observed with the purified extracellular protein B46 from Z. mobilis. These two proteins showed different electrophoretic mobilities and molecular masses and shared no immunological similarly. Thus, the product of sacA (a polypeptide of 58.4-kDa molecular mass) is a new sucrase from Z. mobilis. The amino acid sequence, deduced from the nucleotide sequence of sacA, showed strong homologies with the sucrases from Bacillus subtilis, Salmonella typhimurium, and Vibrio alginolyticus.