Identification of diverse groups of endogenous gammaretroviruses in mega- and microbats
Identification of diverse groups of endogenous gammaretroviruses in mega- and microbats
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DOI：
10.1099/vir.0.043760-0
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发表时间：
2012-09-01
影响因子：
3.8
通讯作者：
Wang, Lin-Fa
中科院分区：
文献类型：
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作者：
Cui, Jie;Tachedjian, Gilda;Wang, Lin-Fa
先前的系统发育研究表明，哺乳动物γ逆转录病毒可能起源于蝙蝠。在这里，我们报告的RNA转录本的发现，从两个假定的内源性γ-逆转录病毒在食果蝙蝠（Rousetus leschenaultii逆转录病毒，RIRV）和食虫蝙蝠（天琴座大皮属逆转录病毒，MIRV）物种。这两种基因组在pol中都有一个大的缺失，表明它们是有缺陷的逆转录病毒。系统发育分析将RIRV和MIRV置于哺乳动物γ逆转录病毒的多样性中，前者更接近猪内源性逆转录病毒，后者更接近Mus dunni内源性病毒、考拉逆转录病毒和巨猿白血病病毒。额外的基因组挖掘表明，无论是microbat（鼠耳蝠lucifugus）和megabat（Pteropus vampyrus）基因组窝藏许多拷贝的内源性逆转录病毒形式有关的RIRV和MIRV。此外，系统发育分析揭示了蝙蝠基因组中存在三组遗传多样性的内源性γ逆转录病毒，M。拥有这三种类型的成员。总而言之，这项研究表明蝙蝠携带不同的γ逆转录病毒，并且可能在哺乳动物γ逆转录病毒多样化过程中作为储存宿主发挥了重要作用。
A previous phylogenetic study suggested that mammalian gammaretroviruses may have originated in bats. Here we report the discovery of RNA transcripts from two putative endogenous gammaretroviruses in frugivorous (Rousettus leschenaultii retrovirus, RIRV) and insectivorous (Megaderma lyra retrovirus, MIRV) bat species. Both genomes possess a large deletion in pol, indicating that they are defective retroviruses. Phylogenetic analysis places RIRV and MIRV within the diversity of mammalian gammaretroviruses, with the former falling closer to porcine endogenous retroviruses and the latter to Mus dunni endogenous virus, koala retrovirus and gibbon ape leukemia virus. Additional genomic mining suggests that both microbat (Myotis lucifugus) and megabat (Pteropus vampyrus) genomes harbour many copies of endogenous retroviral forms related to RIRV and MIRV. Furthermore, phylogenetic analysis reveals the presence of three genetically diverse groups of endogenous gammaretroviruses in bat genomes, with M. lucifugus possessing members of all three groups. Taken together, this study indicates that bats harbour distinct gammaretroviruses and may have played an important role as reservoir hosts during the diversification of mammalian gammaretroviruses.