Plasmodium falciparum culture: The benefits of shaking
Plasmodium falciparum culture: The benefits of shaking
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DOI：
10.1016/j.molbiopara.2009.09.005
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发表时间：
2010-01-01
影响因子：
1.5
通讯作者：
Kirk, Kiaran
中科院分区：
文献类型：
--
作者：
Allen, Richard J. W.;Kirk, Kiaran
尽管有证据表明暂停疟疾培养可以改善寄生虫的生长，但在静态条件下培养寄生虫的做法仍然很普遍。在这里，扩展以前的工作，我们已经量化了连续搅拌对培养物生长的三个指标的有利影响：（i）寄生虫产量，（ii）同步程序后的培养同步性，以及（iii）多种感染的患病率。此外，我们表明，在连续悬浮液下，转基因寄生虫在转染后重新填充培养物所需的时间大大减少。皇冠版权所有（C）2009年出版的爱思唯尔B.V.保留所有权利。
Despite evidence that the suspension of malaria cultures leads to improved parasite growth, the practice of culturing the parasite under static conditions remains widespread. Here, extending previous work, we have quantified the favourable effects of continuous agitation on three indices of culture growth: (i) parasite yield, (ii) culture synchrony after a synchronisation procedure, and (iii) the prevalence of multiple infections. In addition, we show that under continuous suspension, the time taken for genetically altered parasites to re-populate cultures post-transfection is dramatically reduced. Crown Copyright (C) 2009 Published by Elsevier B.V. All rights reserved.