Glycosylphosphatidylinositol-anchored surface molecules of Trypanosoma congolense insect forms are developmentally regulated in the tsetse fly
Glycosylphosphatidylinositol-anchored surface molecules of Trypanosoma congolense insect forms are developmentally regulated in the tsetse fly
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DOI：
10.1016/s0166-6851(01)00382-6
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发表时间：
2002-01-01
影响因子：
1.5
通讯作者：
Roditi, I
中科院分区：
文献类型：
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作者：
Bütikofer, P;Vassella, E;Roditi, I
刚果锥虫的前环培养形式已显示在其表面上表达富含谷氨酸/丙氨酸的蛋白（GARP）。通过标记T.在用糖基磷脂酰肌醇（GPI）前体形成的刚果前环培养物中，我们表明GARP通过GPI锚结合到膜上，并证明存在另外两种大量表达的24-34和58 kDa的GPI锚定的表面分子。24-34 kDa的分子被结合到活锥虫表面的单克隆抗体识别，对蛋白水解具有抗性，这表明它（主要）由非蛋白质物质组成。因此，我们将其命名为抗蛋白酶表面分子（PRS）。与布氏锥虫EP和GPEET原环素相同，T.刚果GPI锚定分子在采采蝇寄生虫发育过程中的变化。感染后不久，PRS在中肠中被前环锥虫大量表达，但在已建立的中肠形式中下调，并且在长鼻中的上鞭毛体形式中完全不存在。相反，GARP在采采蝇侏儒的寄生虫中下调，但在外鞭毛体形式中上调。出乎意料的是，感染后14天，原环形式经常对PRS和GARP都是阴性的，这表明它们可能在生命周期的这一点上表达另一种阶段特异性表面抗原。(C)2002 Elsevier Science B. V.保留所有权利。
Procyclic culture forms of Trypanosoma congolense have been shown to express a glutamic acid/alanine-rich protein (GARP) on their surface. By labelling T. congolense procyclic culture forms with glycosylphosphatidylinositol (GPI) precursors, we show that GARP is bound to the membrane by a GPI anchor and demonstrate the presence of two additional GPI-anchored surface molecules of 24-34 and 58 kDa that are abundantly expressed. The 24-34 kDa molecule, which is recognised by monoclonal antibodies that bind to the surface of living trypanosomes, is resistant to proteolysis, suggesting that it consists (predominantly) of non-proteinaceous material. We have therefore named it protease-resistant surface molecule (PRS). In common with the EP and GPEET procyclins of Trypanosoma brucei, the relative expression of the T. congolense GPI-anchored molecules changes during parasite development in the tsetse fly. PRS is abundantly expressed by procyclic trypanosomes in the midgut shortly after infection, but is downregulated in established midgut forms and completely absent from the epimastigote form in the proboscis. In contrast, GARP is downregulated in parasites in the tsetse fly midget, but upregulated in the epimastigote form. Unexpectedly, 14 days post-infection, procyclic forms frequently are negative for both PRS and GARP, suggesting that they might be expressing another stage-specific surface antigen at this point in the life cycle. (C) 2002 Elsevier Science B.V. All rights reserved.