INCREASES IN CORTICAL DEPTH AND GLIA NUMBERS IN RATS SUBJECTED TO ENRICHED ENVIRONMENT
INCREASES IN CORTICAL DEPTH AND GLIA NUMBERS IN RATS SUBJECTED TO ENRICHED ENVIRONMENT
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DOI：
10.1002/cne.901280110
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发表时间：
1966-01-01
影响因子：
2.5
通讯作者：
BENNETT, EL
中科院分区：
文献类型：
--
作者：
DIAMOND, MC;LAW, F;BENNETT, EL
据报道，生活在丰富刺激环境中的大鼠大脑皮层的解剖和化学变化。目前的研究包括额外的组织学测量，如更广泛的深度测量，差异细胞计数和细胞大小测量。研究人员测量了环境丰富的大鼠和它们贫穷的同伴的视觉皮层的纤维蛋白包埋、硫蛋白染色切片。采用两种方法计算皮质深度：1、眼测微法，在胼胝体两侧各取8个测量值；2、在放大的照片上，每个半球各测量51次，间隔2毫米。用眼测微法，富脑皮层深度比贫脑皮层深度大6.4%。在放大的照片上，富集大鼠的内侧深度比外侧深度增加得更大。在每个横切面上以1.00 mm × 0.76 mm的内侧皮质区域制作重叠显微照片，并合并为每只动物的1张合成图片。由2名技术人员独立确定合成图片中的细胞计数差异。结果显示，在富集的大鼠中，胶质细胞增加了14% （p < 0.01）。用透明照相机绘制的平面计测量的核外周长和核外周长在富裕组和贫困组之间没有发现显著差异。
Anatomical and chemical changes in the cerebral cortex of rats living in an enriched, stimulating environment have been reported. The present study includes additional histological measures such as more extensive depth measures, differential cell counts, and cell size measurements. Celloidin-embedded, thionin-stained sections of the visual cortex from environmentally enriched rats and t leir impoverished littermates were measured. Two methods for calculating cortical depths were used: 1, withan ocular micrometer taking 8 measures lateral to the elevation in the corpus callosum on each hemisphere; 2, on enlarged photographs taking 51 measurements 2 mm apart on each hemisphere. With the ocular micrometer method the cortical depth of the enriched brains was 6.4% greater than the depth of the impoverished cortex. On the enlarged photographs the more medial depths in the enriched rats showed greater increases than the lateral depths. Overlapping photomicrographs were made of a medial cortical area 1.00 mm by 0.76 mm on each transverse section and were combined into 1 composite picture for each animal. Differential cell counts from the composite pictures were determined independently by 2 technicians. Results indicate a 14% (p [less than] 0.01) increase in glia among the enriched rats. No significant differences between enriched and Impoverished groups were found in the perikarya and nuclear circumferences as measured with a planimeter from camera lucida drawings.