CELLULAR HOMOLOG (C-SRC) OF THE TRANSFORMING GENE OF ROUS-SARCOMA VIRUS - ISOLATION, MAPPING, AND TRANSCRIPTIONAL ANALYSIS OF C-SRC AND FLANKING REGIONS
CELLULAR HOMOLOG (C-SRC) OF THE TRANSFORMING GENE OF ROUS-SARCOMA VIRUS - ISOLATION, MAPPING, AND TRANSCRIPTIONAL ANALYSIS OF C-SRC AND FLANKING REGIONS
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DOI：
10.1073/pnas.78.9.5842
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发表时间：
1981-01-01
期刊：
影响因子：
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通讯作者：
BISHOP, JM
中科院分区：
文献类型：
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作者：
PARKER, RC;VARMUS, HE;BISHOP, JM
Rous肉瘤病毒的致瘤特性归因于一种由单一病毒基因v-src编码的60,000道尔顿蛋白pp60v-src。在所有测试的脊椎动物细胞中都发现了一个同源基因c-src，它包含一个60,000道尔顿蛋白pp60c-src的信息。通过筛选代表鸡基因组的重组DNA文库，分离到2个重叠的DNA片段，它们包含跨越pp60c-src编码序列的30kb的DNA。这个30kb的区域没有中等或高度重复的序列，与整个病毒基因和v-src的5‘’非编码序列有同源性。尽管v-src具有不间断的编码序列，但c-src被最少7个插入序列中断。在分离的DNA中似乎至少编码了2个多聚腺苷酸化的RNA。更大的RNA，.apprx。3.9kb，是推测的c-src mRNA；.apprx较小的转录本。2kb与v-src/c-src同源区3‘端的几千个碱基的DNA序列杂交。
The tumorigenic properties of Rous sarcoma virus are attributable to a 60,000-dalton protein, pp60v-src, encoded by a single viral gene, v-src. A homologous gene, c-src, that contains the information for a 60,000-dalton protein, pp60c-src, was identified in all tested vertebrate cells. By screening a recombinant DNA library representative of the chicken genome, 2 overlapping DNA fragments that contain > 30 kilobases (kb) of DNA spanning the coding sequences for pp60c-src were isolated. This 30-kb region is devoid of moderately or highly repeated sequences and shares homology with the entire viral gene and noncoding sequences 5'' of v-src. Although v-src has an uninterrupted coding sequence, c-src is interrupted by a minimum of 7 intervening sequences. At least 2 polyadenylylated RNA seem to be encoded within the isolated DNA. The larger RNA, .apprx. 3.9 kb, is the presumptive c-src mRNA; the smaller transcript of .apprx. 2 kb hybridizes to DNA sequences several kilobases from the 3'' end of the v-src/c-src homology area.