Changes in the genetic diversity of barley of Nordic and Baltic origin, studied by isozyme electrophoresis.
Changes in the genetic diversity of barley of Nordic and Baltic origin, studied by isozyme electrophoresis.
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DOI：
10.1079/pgr2003021
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发表时间：
2003-01-01
期刊：
影响因子：
--
通讯作者：
von Bothmer, R.
中科院分区：
文献类型：
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作者：
Brantestam, A. K.;Bothmer, R. von;von Bothmer, R.
在这项研究中，对源自北欧和波罗的海的大麦 (Hordeum vulgare L.) 从 19 世纪末至今的遗传变异变化进行了评估。还对北欧和波罗的海材料与未经严格选择的奇异材料进行了比较。使用同工酶淀粉凝胶电泳对总共 293 个种质进行了调查，其中包括 160 个北欧和 80 个波罗的海地方品种、栽培品种和育种系，以及另外 53 个外来起源（中亚）的地方品种。对于所研究的四种同工酶，在 11 个基因座观察到 28 个等位基因。各个位点的平均总遗传多样性值 (HT) 范围在 0 到 0.519 之间。在外来材料中，有九个位点是多态性的，两个是单态性的，而北欧和波罗的海材料中则有七个多态性和四个单态性位点。一些罕见的等位基因仅在外来材料中检测到。对北欧和波罗的海材料的同工酶位点研究表明，与地方品种、古老和外来材料相比，现代品种的平均遗传多样性值较低。
In this study an evaluation was made of changes in the genetic variation of barley (Hordeum vulgare L.) of Nordic and Baltic origin, from the end of the 19th century until today. A comparison of Nordic and Baltic material with exotic material that has not been subjected to intense selection was also made. A total of 293 accessions, including 160 Nordic and 80 Baltic landraces, cultivars and breeding lines, and another 53 landraces of exotic origin (Central Asia), were surveyed using isozyme starch gel electrophoresis. For the four isozymes studied, 28 alleles at 11 loci were observed. The average total genetic diversity value (HT) for individual loci ranged between 0 and 0.519. In the exotic material nine loci were polymorphic and two monomorphic, compared to seven polymorphic and four monomorphic loci in the Nordic and Baltic material. Some of the rare alleles were detected only in the exotic material. The studied isozyme loci of Nordic and Baltic material indicated that modern cultivars have a lower average genetic diversity value compared to the landraces, old and exotic material.