The in situ structures of mono-, di-, and trinucleosomes in human heterochromatin.
The in situ structures of mono-, di-, and trinucleosomes in human heterochromatin.
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DOI：
10.1091/mbc.e18-05-0331
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发表时间：
2018-10-01
影响因子：
3.3
通讯作者：
Gan L
中科院分区：
文献类型：
--
作者：
Cai S;Böck D;Pilhofer M;Gan L
染色质在核小体和核小体水平上的原位三维组织尚不清楚。在这里，我们使用冷冻电子断层扫描，以确定原位结构的HeLa核小体，它具有典型的核心结构和不对称的，灵活的接头DNA。亚体层图像重定位表明异染色质中的连续核小体在染色体水平上遵循不规则的路径。这种高级抑制性染色质折叠的基本原理与单核小体水平上两个连接DNA的构象变异性是相容的。
The in situ three-dimensional organization of chromatin at the nucleosome and oligonucleosome levels is unknown. Here we use cryo-electron tomography to determine the in situ structures of HeLa nucleosomes, which have canonical core structures and asymmetric, flexible linker DNA. Subtomogram remapping suggests that sequential nucleosomes in heterochromatin follow irregular paths at the oligonucleosome level. This basic principle of higher-order repressive chromatin folding is compatible with the conformational variability of the two linker DNAs at the single-nucleosome level.