Antioxidant and DNA Protection Effects of Taurine by Electron Spin Resonance Spectroscopy
Antioxidant and DNA Protection Effects of Taurine by Electron Spin Resonance Spectroscopy
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DOI：
10.1007/978-1-4614-6093-0_17
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发表时间：
2013-01-01
期刊：
影响因子：
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通讯作者：
Chang, Kyung Ja
中科院分区：
文献类型：
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作者：
Cheong, Sun Hee;Moon, Sang Ho;Chang, Kyung Ja
牛磺酸可能在保护细胞免受抗氧化剂的毒性损伤方面发挥重要作用。然而，缺乏证据支持这一假设。本研究的目的是研究在体外抗氧化性能的牛磺酸对不同的反应物种在不同的浓度。采用自旋捕集电子方法研究了牛磺酸对DPPH自由基、羟自由基、超氧阴离子自由基和烷基自由基的清除作用，并与电子自旋共振（ESR）信号强度进行了比较。ESR检测结果表明，不同浓度（0.0625与1 mg/mL相近）牛磺酸清除DPPH自由基的活性随ESR信号减弱而增强，并呈剂量依赖性。牛磺酸对羟自由基、超氧阴离子自由基和烷基自由基均有清除作用。这项研究的结果表明，牛磺酸可能是一个有用的自由基清除剂和一个潜在的补充食品，制药和化妆品行业以及饲料和/或抗生素，因为它对各种活性自由基的有效的抗氧化能力。
Taurine may play an important role in protecting cells against toxic injury by an antioxidant. However, there is a lack of evidence to support this hypothesis. The objective of this study was to examine the in vitro antioxidant properties of taurine against different reactive species at various concentrations. The radical scavenging effects of taurine on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, hydroxyl radical, superoxide radical, and alkyl radical were investigated using a spin-trapping electron method and compared with the electron spin resonance (ESR) signal intensity. ESR assays showed that DPPH radical scavenging activity of taurine at various concentrations (0.0625 similar to 1 mg/mL) was elevated with a decrease of ESR signals in a dose-dependent manner. Moreover, taurine exhibited the radical scavenging activities against hydroxyl radicals, superoxide radicals, and alkyl radicals. Findings from this study suggest that taurine may be a useful radical scavenger and a potential supplement for the food, pharmaceutical, and cosmetic industries as well as feed and/or antibiotic because of its potent antioxidant capacities against various reactive radicals.