Effects of inhalational anaesthetics in experimental allergic asthma
Effects of inhalational anaesthetics in experimental allergic asthma
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DOI：
10.1111/anae.12593
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发表时间：
2014-06-01
期刊：
影响因子：
10.7
通讯作者：
Rocco, P. R. M.
中科院分区：
文献类型：
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作者：
Burburan, S. M.;Silva, J. D.;Rocco, P. R. M.
我们评估了异氟烷、氟烷和七氟烷是否能减轻实验性哮喘的炎症反应并改善肺形态功能。用卵清蛋白致敏和攻击 56 只 BALB/c 小鼠，并用异氟烷、氟烷、七氟烷或戊巴比妥钠麻醉一小时。评估肺力学和组织学。分析了促炎症（肿瘤坏死因子）、促纤维形成（转化生长因子）和促血管生成（血管内皮生长因子）介质以及氧化过程调节剂的基因表达。这些调节剂包括核因子 erythroid-2 相关因子 2、sirtuin、过氧化氢酶和谷胱甘肽过氧化物酶。与戊巴比妥钠相比，异氟烷、氟烷和七氟烷可降低气道阻力、静态肺弹性和肺不张。七氟烷可最大限度地减少支气管收缩和细胞浸润，并减少肿瘤坏死因子、转化生长因子、血管内皮生长因子、去乙酰化酶、过氧化氢酶和谷胱甘肽过氧化物酶，同时增加核因子红细胞 2 相关因子 2 的表达。七氟烷下调炎症、纤维生成和血管生成介质，调节氧化-抗氧化失衡，改善哮喘模型的肺功能。
We evaluated whether isoflurane, halothane and sevoflurane attenuate the inflammatory response and improve lung morphofunction in experimental asthma. Fifty-six BALB/c mice were sensitised and challenged with ovalbumin and anaesthetised with isoflurane, halothane, sevoflurane or pentobarbital sodium for one hour. Lung mechanics and histology were evaluated. Gene expression of pro-inflammatory (tumour necrosis factor-), pro-fibrogenic (transforming growth factor-) and pro-angiogenic (vascular endothelial growth factor) mediators, as well as oxidative process modulators, were analysed. These modulators included nuclear factor erythroid-2 related factor 2, sirtuin, catalase and glutathione peroxidase. Isoflurane, halothane and sevoflurane reduced airway resistance, static lung elastance and atelectasis when compared with pentobarbital sodium. Sevoflurane minimised bronchoconstriction and cell infiltration, and decreased tumour necrosis factor-, transforming growth factor-, vascular endothelial growth factor, sirtuin, catalase and glutathione peroxidase, while increasing nuclear factor erythroid-2-related factor 2 expression. Sevoflurane down-regulated inflammatory, fibrogenic and angiogenic mediators, and modulated oxidant-antioxidant imbalance, improving lung function in this model of asthma.