How should the optical tweezers experiment be used to characterize the red blood cell membrane mechanics?
How should the optical tweezers experiment be used to characterize the red blood cell membrane mechanics?
复制标题
DOI：
10.1007/s10237-017-0910-x
复制
发表时间：
2017-05
影响因子：
3.5
通讯作者：
J. Sigüenza;S. Mendez;F. Nicoud
中科院分区：
文献类型：
--
作者：
J. Sigüenza;S. Mendez;F. Nicoud
使用光学镊子拉伸红细胞是一种通过测量细胞沿拉伸方向(轴向直径)和垂直方向(横向直径)的大小来表征其膜的机械性能的方法。最近，这样的数据已经在许多出版物中被用来验证致力于计算流动下的红细胞动力学的解算器。在本研究中，使用不同的力学模型来模拟光镊子对红细胞的拉伸。结果首先表明，膜的机械模数必须根据所使用的模型进行调整。此外，通过评估细胞的面积扩张，在光钳实验中测量的轴向和横向直径被发现不足以区分与红细胞相关的模型。结果表明，由于其它参数对膜模型的敏感性较高，因此应优先考虑其他参数，如电池的高度或轮廓。
Stretching red blood cells using optical tweezers is a way to characterize the mechanical properties of their membrane by measuring the size of the cell in the direction of the stretching (axial diameter) and perpendicularly (transverse diameter). Recently, such data have been used in numerous publications to validate solvers dedicated to the computation of red blood cell dynamics under flow. In the present study, different mechanical models are used to simulate the stretching of red blood cells by optical tweezers. Results first show that the mechanical moduli of the membranes have to be adjusted as a function of the model used. In addition, by assessing the area dilation of the cells, the axial and transverse diameters measured in optical tweezers experiments are found to be insufficient to discriminate between models relevant to red blood cells or not. At last, it is shown that other quantities such as the height or the profile of the cell should be preferred for validation purposes since they are more sensitive to the membrane model.