Polysaccharide-polynucleotide complexes, Part 18. Rod-like architecture and helicity of the poly(C)/schizophyllan complex observed by AFM and SEM
Polysaccharide-polynucleotide complexes, Part 18. Rod-like architecture and helicity of the poly(C)/schizophyllan complex observed by AFM and SEM
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DOI：
10.1016/j.carres.2003.09.032
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发表时间：
2004-01-22
影响因子：
3.1
通讯作者：
Sakurai, K
中科院分区：
文献类型：
--
作者：
Bae, AH;Lee, SW;Sakurai, K
采用AFM和SEM对聚（C）和SPG之间的复合物进行了显微镜研究，发现该复合物在云母表面上具有与复性SPG和SPG的原始三股螺旋相同的棒状结构，表明该复合物也具有螺旋结构。SEM观察结果显示，仅当样品被金属遮蔽时，棒表面上才出现螺旋图案。从图像中评估的间距与从晶体学数据中获得的间距相当。螺旋结构可视化的能力可以从铂颗粒可以使用SPG（或复合物）的分子表面作为模板在样品上组装的假设来解释。(C)2003 Elsevier Ltd.保留所有权利。
Microscopic studies of the complex between poly(C) and schizophyllan (SPG), employing both AFM and SEM, revealed that the complex takes the same rod-like architecture on the mica surface as those of the renatured SPG and the original triple helix of SPG, indicating that the complex also has a helical structure. The SEM observations showed the helical pattern on the rod surface, only when the sample was metal shadowed. The pitch evaluated from the image is comparable with that obtained from crystallographic data. The ability to visualize the helical structure can be explained from the hypothesis that the platinum grains may assemble on the sample using the molecular surface of the SPG (or complex) as the template. (C) 2003 Elsevier Ltd. All rights reserved.