Real-Time Reverse Transcription PCR
Real-Time Reverse Transcription PCR
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DOI：
10.3109/9780203997352.226
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发表时间：
2005
期刊：
影响因子：
--
通讯作者：
S. Bustin
中科院分区：
文献类型：
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作者：
S. Bustin
基于荧光的实时逆转录聚合酶链反应（RT-PCR）已从实验技术转变为检测RNA的主流科学工具。这是因为以下几个因素：1）它是一种均相检测试剂盒，无需PCR后处理; 2）它具有较宽的动态范围; 3）检测试剂盒间差异很小; 4）它实现了基于PCR的检测试剂盒的固有定量能力，使其成为定量而非定性检测试剂盒。这些性质符合分子医学中对定量数据的明显要求（例如，用于测量病毒载量、监测癌症中的隐匿性疾病、或通过药物基因组学检查个体对治疗的反应的遗传基础）。
Real-time, fluorescence-based reverse transcription polymerase chain reaction (RT-PCR) has been transformed from an experimental technology into a mainstream scientific tool for the detection of RNA. This is because of several factors: 1) it is a homogeneous assay, which eliminates the requirement for post-PCR processing; 2) it has a wide dynamic range; 3) there is little interassay variation; and 4) it realizes the inherent quantitative capacity of PCR-based assays, making it a quantitative, rather than a qualitative, assay. These properties match the evident requirement in molecular medicine for quantitative data (e.g., for measuring viral load, monitoring of occult disease in cancer, or examining the genetic basis for individual variation in response to therapeutics through pharmacogenomics).