Kupffer cells are a dominant site of uncoupling protein 2 expression in rat liver
Kupffer cells are a dominant site of uncoupling protein 2 expression in rat liver
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DOI：
10.1006/bbrc.1997.6852
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发表时间：
1997-06-27
影响因子：
3.1
通讯作者：
Ricquier, D
中科院分区：
文献类型：
--
作者：
Larrouy, D;Laharrague, P;Ricquier, D
肝脏产热的机制尚不清楚，可能与棕色脂肪细胞线粒体解偶联蛋白（UCP 1）相关。本文证明，UCP 1在大鼠的任何肝细胞类型中不表达，而UCP 2，最近克隆的UCP 1的同源物，在枯否细胞中以非常高的水平表达，但不在肝细胞中。这种高水平的表达UCP2在枯否细胞允许交叉免疫反应性抗体直接针对UPC 1。在表达大鼠UCP2的转染酵母的Western印迹分析中，通过用抗UCP1抗体检测UCP2证实了这种交叉反应性。UCP2在枯否细胞中的高水平表达表明UCP2在巨噬细胞中的特定功能。(C)北京：科学出版社.
The mechanisms underlying thermogenesis in liver are not well understood, They may involve proteins related to the mitochondrial uncoupling protein (UCP1) of brown adipocytes. In this paper, it is demonstrated that UCP1 is not expressed in any liver cell type of rat while UCP2, a recently cloned homologue of UCP1, is expressed at a very high level in Kupffer cells but not in hepatocytes. This high level of expression of UCP2 in Kupffer cells allowed cross immunoreactivity with antibodies directed against UPC1. This cross reactivity was confirmed by the detection of UCP2 with anti-UCP1 antibody, in western blotting analysis of transfected yeasts expressing rat UCP2. The high level expression of UCP2 in Kupffer cells suggests a particular function of UCP2 in macrophages. (C) 1997 Academic Press.