Changes in the Distribution of Nerve Fibers Immunoreactive to Calcitonin Gene-Related Peptide According to Growth and Aging in Rat Molar Periodontal Ligament
Changes in the Distribution of Nerve Fibers Immunoreactive to Calcitonin Gene-Related Peptide According to Growth and Aging in Rat Molar Periodontal Ligament
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DOI：
10.2319/040109-185.1
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发表时间：
2010-03-01
影响因子：
3.4
通讯作者：
Soma, Kunimichi
中科院分区：
文献类型：
--
作者：
Jung, Hangsul;Horiuchi, Mariko;Soma, Kunimichi
目的：分析降钙素基因相关肽(CGRP-ir)免疫反应神经纤维在大鼠牙周韧带中的增龄变化。材料与方法：30只雄性Wistar-ST大鼠随机分为生长期(5、9、15周龄)和增龄组(6、12、24月龄)，每组5只。与上颌右第一磨牙远中颊根平行切取8个连续矢状面，厚5µm。用兔抗CGRP的单抗对这些组织进行染色。结果：CGRP-ir神经纤维主要分布于根尖周膜，纤维数量明显多于老年组。结论：CGRP-ir神经纤维在牙周膜中密度较大，随着年龄的增长逐渐减少，提示CGRP可能影响牙周组织的生长和衰老。(角度直角。2010；80：309-315。)
Objective: To analyze the age-dependent changes in nerve fibers immunoreactive to calcitonin gene-related peptide (CGRP-ir) in the periodontal ligaments of rats.Materials and Methods: Thirty male Wistar-ST rats were divided into growing groups (5, 9, and 15 weeks of age) and aging groups (6, 12, and 24 months of age) (n = 5 in each group). Eight serial sagittal sections, 5 mu m thick, were cut parallel to the distobuccal root of the maxillary right first molar. These tissues were stained with a rabbit monoclonal antibody against CGRP. The observation area was divided into three parts (mesial, apical, and distal) and observed using a light microscope.Results: CGRP-ir nerve fibers were primarily distributed in the apical periodontal ligament in the growing group, with significantly more fibers than in the aging group.Conclusions: CGRP-ir nerve fibers in the periodontal ligament are dense during the growth period and decrease gradually with aging, indicating that CGRP may affect periodontal tissue with growth and aging. (Angle Orthod. 2010;80:309-315.)