Imaging molecular expression on vascular endothelial cells by in vivo immunofluorescence microscopy
Imaging molecular expression on vascular endothelial cells by in vivo immunofluorescence microscopy
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DOI：
10.2310/7290.2006.00004
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发表时间：
2006-01-01
影响因子：
2.8
通讯作者：
Lin, Charles P.
中科院分区：
文献类型：
--
作者：
Runnels, Judith M.;Zamiri, Parisa;Lin, Charles P.
血管内皮上的分子表达对于调节循环细胞与血管壁的相互作用至关重要。白细胞以及循环癌细胞通过与内皮细胞 (EC) 上的粘附分子相互作用而进入组织。人们越来越多地探索 EC 上的分子靶标，以用于治疗剂和显像剂的组织特异性递送。在这里，我们使用体内免疫荧光显微镜来观察活体动物脉管系统中内皮分子的表达。在用荧光抗体原位标记 EC 表面标记后，使用体内共焦和多光子显微镜对完整皮肤进行光学切片，获得高分辨率图像。其他血管床（例如骨髓和眼部血管）可以在很少或无需组织操作的情况下进行成像。实时成像对于跟踪炎症反应期间 E-选择素等诱导分子的动态表达特别有用。
Molecular expression on the vascular endothelium is critical in regulating the interaction of circulating cells with the blood vessel wall. Leukocytes as well as circulating cancer cells gain entry into tissue by interacting with adhesion molecules on the endothelial cells (EC). Molecular targets on the EC are increasingly being explored for tissue-specific delivery of therapeutic and imaging agents. Here we use in vivo immunofluorescence microscopy to visualize the endothelial molecular expression in the vasculature of live animals. High-resolution images are obtained by optical sectioning through the intact skin using in vivo confocal and multiphoton microscopy after in situ labeling of EC surface markers with fluorescent antibodies. Other vascular beds such as the bone marrow and ocular blood vessels can be imaged with little or no tissue manipulation. Live imaging is particularly useful for following the dynamic expression of inducible molecules such as E-selectin during an inflammatory response.