Distribution of tissue progenitors within the shield region of the zebrafish gastrula.
Distribution of tissue progenitors within the shield region of the zebrafish gastrula.
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作者：
J. Shih;S. Fraser
斑马鱼已经成为脊椎动物发育实验分析的重要模型系统，因为它可以进行遗传分析，而且它的光学清晰度允许在体内跟踪单个细胞的运动和分化。在这篇文章中，我们试图描述组织祖细胞在早期原肠胚胚胎屏蔽区外层细胞内的空间分布。采用离子导入法对单个细胞进行荧光标记。随后，我们记录了他们关于胚胎盾牌的位置和他们最终的命运。我们的数据显示，神经、脊索、躯体和内胚层谱系的祖细胞都存在于胚胎屏蔽区，并且这些祖细胞以混合种群的形式排列。此外，在中线附近，有证据表明各层之间的神经和脊索前体细胞的分布存在显著的偏差，这表明斑马鱼胚胎屏蔽区内存在一定程度的放射状组织。组织祖细胞在斑马鱼原肠胚中的分布与在两栖动物中的分布显著不同，这不仅关系到对突变表型和原位染色模式的解释，也关系到我们对斑马鱼原肠发育过程中的形态发生运动和神经诱导的理解。
The zebrafish has emerged as an important model system for the experimental analysis of vertebrate development because it is amenable to genetic analysis and because its optical clarity allows the movements and the differentiation of individual cells to be followed in vivo. In this paper, we have sought to characterize the spatial distribution of tissue progenitors within the outer cell layers of the embryonic shield region of the early gastrula. Single cells were labeled by iontophoretic injection of fluorescent dextrans. Subsequently, we documented their position with respect to the embryonic shield and their eventual fates. Our data show that progenitor cells of the neural, notochordal, somitic and endodermal lineages were all present within the embryonic shield region, and that these progenitors were arranged as intermingled populations. Moreover, close to the midline, there was evidence for significant biases in the distribution of neural and notochord progenitors between the layers, suggesting some degree of radial organization within the zebrafish embryonic shield region. The distributions of tissue progenitors in the zebrafish gastrula differ significantly from those in amphibians; this bears not only on interpretations of mutant phenotypes and in situ staining patterns, but also on our understanding of morphogenetic movements during gastrulation and of neural induction in the zebrafish.