Differential Expression of Sphingosine-1-Phosphate Receptors 1-5 in the Developing Nervous System
Differential Expression of Sphingosine-1-Phosphate Receptors 1-5 in the Developing Nervous System
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DOI：
10.1002/dvdy.21852
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发表时间：
2009-02-01
影响因子：
2.5
通讯作者：
Lee, V. M.
中科院分区：
文献类型：
--
作者：
Meng, H.;Lee, V. M.
1-磷酸鞘氨醇（SIP）与G蛋白偶联受体结合，可调节多种细胞功能。在以前的研究中，我们分离出两个关键酶的SIP途径中表达的迁移神经嵴细胞。为了确定SIP受体是否存在于神经嵴细胞或周围神经系统中，我们检测了SIP受体（S1 pr 1 -5）在小鼠中的表达模式，以及S1 pr 1和S1 pr 3在鸡胚中的表达模式。在这里，我们提出了一个全面的表达分析，这些受体使用原位杂交，提供时空信息。我们发现，S1 pr 2表达在迁移的颅神经嵴细胞和肠神经元。S1 pr 1主要表达于神经上皮，而S1 pr 4和S1 pr 5则在后期的神经元中表达。相反，S1 pr 3主要在神经结构内和周围的非神经元细胞中检测到。我们还描述了SIP受体在发育中的神经系统中的新表达位点。发展动力学238：487-500，2009年。(c)2009威利-利斯公司
Sphingosine-1-phosphate (SIP) binds to G protein-coupled receptors and can regulate a wide range of cellular functions. In a previous study, we isolated two key enzymes in the SIP pathway that were expressed in migrating neural crest cells. To determine if SIP receptors are present in neural crest cells or peripheral nervous system, we examine the expression patterns of SIP receptors (S1pr1-5) in mouse, and s1pr1 and s1pr3 in chick embryos. Here, we present a comprehensive expression analysis of these receptors using in situ hybridizations, which provide spatiotemporal information. We showed that S1pr2 was expressed in migrating cranial neural crest cells and enteric neurons. S1pr1 was prominently expressed in the neuroepithelium whereas S1pr4 and S1pr5 were in neurons at later stages. On the contrary, S1pr3 was predominantly detected in non-neuronal cells within and surrounding neural structures. We also described novel expression sites for SIP receptors in the developing nervous system. Developmental Dynamics 238:487-500, 2009. (c) 2009 Wiley-Liss, Inc.