Differences in mineral accumulation and gene expression profiles between two metal hyperaccumulators, Noccaea japonica and Noccaea caerulescens ecotype Ganges, under excess nickel condition
Differences in mineral accumulation and gene expression profiles between two metal hyperaccumulators, Noccaea japonica and Noccaea caerulescens ecotype Ganges, under excess nickel condition
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过量镍条件下两种金属超富集植物Noccaea japonica和Noccaea caerulescens生态型恒河之间矿物质积累和基因表达谱的差异
DOI：
10.1080/15592324.2021.1945212
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发表时间：
2021
影响因子：
2.9
通讯作者：
Nishida Sho
中科院分区：
文献类型：
--
作者：
Enomoto Takuo;Yoshida Junko;Mizuno Takafumi;Watanabe Toshihiro;Nishida Sho
本研究比较了两种金属超富集植物——产自富镍蛇纹土壤的日本野菜和产自锌/铅矿土壤的绿绿野菜（生态型）——在过量镍条件下的矿物积累和基因表达模式。K、P、Mg、B和Mo积累的显著差异可以通过每种矿物质特定转运蛋白的表达水平来解释。我们之前的研究表明，整个植株的总Ni积累量更高。caerulescensthan客栈。粳稻。在这里，我们发现铁在过量Ni下有类似的趋势；然而，铁调控转运蛋白1（IRT1）的表达较高，IRT1编码主要铁摄取转运蛋白并导致拟南芥过量的镍摄取。粳稻。NjIRT1在Asp100位点有一个点突变，这是铁转运所必需的，因此可能缺乏其铁和镍转运功能。日本野菜可能已经失去了IRT1的功能，这种功能可以防止在富含镍的土壤中通过IRT1过量吸收镍，并依赖于其他转运蛋白。
Here we compare mineral accumulation and global gene expression patterns between two metal hyperaccumulator plants –Noccaea japonica, originating from Ni-rich serpentine soils, andNoccaea caerulescens(ecotype Ganges), originating from Zn/Pb-mine soils – under excess Ni conditions. Significant differences in the accumulation of K, P, Mg, B, and Mo were explained by the expression levels of specific transporters for each mineral. We previously showed that total Ni accumulation in the whole plant is higher inN. caerulescensthan inN. japonica. Here we found a similar tendency for Fe under excess Ni; however, the expression ofiron-regulated transporter 1(IRT1), which encodes the primary Fe uptake transporter and causes excess Ni uptake inArabidopsis thaliana, was higher inN. japonica. NjIRT1 has a point mutation at Asp100, which is essential for Fe transport, and so might lack its Fe and possibly Ni transport function.Noccaea japonicamight have lost its IRT1 function, which would prevent excess Ni uptake via IRT1 in Ni-rich soils, and come to rely on other transporters.