High-sensitivity cytometric detection using fluidic-photonic integrated circuits with array waveguides
High-sensitivity cytometric detection using fluidic-photonic integrated circuits with array waveguides
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DOI：
10.1109/jstqe.2005.857743
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发表时间：
2005-07-01
影响因子：
4.9
通讯作者：
Lo, YH
中科院分区：
文献类型：
--
作者：
Lien, V;Zhao, K;Lo, YH
我们展示了一种用于微型制造的流式细胞仪的新的检测方案。执行流式细胞仪检测的流体-光子集成电路(FPIC)具有新的功能，如片上激励、飞行时间测量，最重要的是，大大增强了荧光检测的灵敏度。利用空分波导解复用器的结构和互相关分析技术，在不需要大功率激光激励和锁相放大器(或光电倍增管)检测的情况下，用简单的光源和探测器获得了高检测灵敏度。除了改进细胞检测，将微流控电路与光子电路集成到FPIC中的技术为复杂的生物医学传感设备提供了一个新的平台，具有显著的成本、尺寸和性能优势。
We demonstrate a new detection scheme for a micro-fabricated flow cytometer. The fluidic-photonic integrated circuits (FPICs) that perform flow cytometric detection possess new functionality, such as on-chip excitation, time-of-flight measurement, and above all, greatly enhanced fluorescence detection sensitivity. Using the architecture of space-division waveguide demultiplexer and the technique of cross-correlation analysis, we obtained high detection sensitivity with a simple light source and a detector, without high-power laser excitation and lock-in amplifier (or photomultiplier tube) detection. Besides improving cytometric detection, the technology of integrating microfluidic circuits with photonic circuits into the FPIC presents a new platform for sophisticated biomedical-sensing devices with significant cost, size, and performance advantages.