Hydrophobic Gold Nanoparticle Self-Assembly with Phosphatidylcholine Lipid: Membrane-Loaded and Janus Vesicles
Hydrophobic Gold Nanoparticle Self-Assembly with Phosphatidylcholine Lipid: Membrane-Loaded and Janus Vesicles
复制标题
DOI：
10.1021/nl102387n
复制
发表时间：
2010-09-01
期刊：
影响因子：
10.8
通讯作者：
Korgel, Brian A.
中科院分区：
文献类型：
--
作者：
Rasch, Michael R.;Rossinyol, Emma;Korgel, Brian A.
通过低温透射电子显微镜（cryoTEM）研究了直径小于2 nm的疏水性十二硫醇包覆的Au纳米颗粒和磷脂酰胆碱（PC）脂质囊泡的挤出杂交。纳米颗粒将囊泡作为致密的单层装载在脂质双层的疏水核心中，而不破坏它们的结构。纳米颗粒-囊泡杂化物也可以通过透析过程制备，将预先形成的囊泡与洗涤剂稳定的纳米颗粒混合，但这种方法导致囊泡仅部分负载有分离成半球形域的纳米颗粒，形成Janus囊泡-纳米颗粒杂化结构。
Hybrids of hydrophobic sub-2-nm-diameter dodecanethiol-coated Au nanoparticles and phosphatidylcholine (PC) lipid vesicles made by extrusion were examined by cryogenic transmission electron microscopy (cryoTEM). The nanoparticles loaded the vesicles as a dense monolayer in the hydrophobic core of the lipid bilayer, without disrupting their structure. Nanoparticle-vesicle hybrids could also be made by a dialysis process, mixing preformed vesicles with detergent stabilized nanoparticles, but this approach led to vesicles only partially loaded with nanoparticles that segregated into hemispherical domains forming a Janus vesicle-nanoparticle hybrid structure.