Identification of two novel human dynein light chain genes, DNLC2A and DNLC2B, and their expression changes in hepatocellular carcinoma tissues from 68 Chinese patients
Identification of two novel human dynein light chain genes, DNLC2A and DNLC2B, and their expression changes in hepatocellular carcinoma tissues from 68 Chinese patients
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DOI：
10.1016/s0378-1119(01)00787-9
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发表时间：
2001-12-27
期刊：
影响因子：
3.5
通讯作者：
Zhao, SY
中科院分区：
文献类型：
--
作者：
Jiang, JM;Yu, L;Zhao, SY
两个全长cdna。克隆并鉴定了DNLC2A和DNLC2B。它们的开放阅读框分别编码96个氨基酸，与在线虫、果蝇、小鼠和大鼠中保守的古老动力蛋白轻链家族成员之一的路障/LC7最同源。DNLC2A在16个人体组织中有12个组织表达，其中在心脏、肝脏和大脑中表达较强，而在肺、前列腺、睾丸、小肠和结肠中表达较弱。除肝脏外，DNLC2B的表达普遍高于DNLC2A。Northern blotting和/或半定量RT-PCR检测了68例肝癌组织样本中DNLC2A和DNLC2B的表达变化。结果显示，与邻近无肿瘤肝组织相比，DNLC2A上调（68例中45例），DNLC2B下调（68例中44例）。有趣的是，在68例肝癌样本中，28例DNLC2A上调，而DNLC2B下调；10例患者DNLC2A表达上调，DNLC2B表达无明显变化；14例患者DNLC2A表达无明显变化，DNLC2B表达下调。尽管潜在的机制尚不清楚，但DNLC2A的明显上调和DNLC2B的下调表明这些基因可能参与了肿瘤的进展。另一方面，两种同源基因的不同表达变化表明肝细胞癌是由不同的病理机制引起的。此外，通过辐射杂交定位将DNLC2A定位到人类染色体20q12-q13.11标记D20S106附近。(C) 2001年Elsevier Science B.V.出版
Two full-length cDNAs. DNLC2A and DNLC2B, were cloned and characterized. Their open reading frames respectively encode 96 amino acids which are most closely homologous to roadblock/LC7, one member of an ancient dynein light chain protein family, conserved in nematode, fruit fly, mouse and rat. The DNLC2A was expressed in 12 of 16 human tissues examined, with especially strong expression in heart, liver and brain, whereas there was weak expression in lung, prostrate, testis, small intestine and colon. The expression of DNLC2B was generally high compared with that of DNLC2A except in liver. Northern blotting and/or semi-quantitative RT-PCR analysis examined the expression changes of DNLC2A and DNLC2B in 68 hepatocellular carcinoma tissue samples. It was revealed that DNLC2A was up-regulated (45 out of the 68 cases) while DNLC2B was down-regulated (44 out of 68 cases), compared with their adjacent tumor-free liver tissues. Interestingly, among the total 68 liver cancer samples tested, DNLC2A was up-regulated while DNLC2B was down-regulated in 28 cases; DNLC2A was up-regulated while no obvious change was observed for DNLC2B in 10 cases; no obvious change was observed for DNLC2A while DNLC2B was down-regulated in 14 cases. Although the underlying mechanism is not clear to date, the apparent up-regulation of DNLC2A and down-regulation of DNLC2B suggest that these genes might be involved in tumor progression. On the other hand, the different expression changes of the two homologous genes indicate that hepatocellular carcinomas are caused by different pathological mechanisms. In addition, DNLC2A was assigned to human chromosome 20q12-q13.11 near the marker D20S106 by radiation hybrid mapping. (C) 2001 Published by Elsevier Science B.V.