Toxicity of oral cadmium intake: Impact on gut immunity
Toxicity of oral cadmium intake: Impact on gut immunity
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DOI：
10.1016/j.toxlet.2015.06.002
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发表时间：
2015-09-02
影响因子：
3.5
通讯作者：
Kataranovski, Milena
中科院分区：
文献类型：
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作者：
Ninkov, Marina;Aleksandrov, Aleksandra Popov;Kataranovski, Milena
胃肠道是镉（Cd）污染的主要靶器官之一。在本研究中，亚慢性（30天）口服（在水中）摄入5 ppm和50 ppm的镉对肠道免疫反应的影响进行了研究。镉消耗导致乳酸杆菌菌株对应的细菌减少、组织损伤和肠道炎症[高迁移率族蛋白1（HMGB 1分子）、超氧化物歧化酶（SOD）和过氧化氢酶（CAT）活性以及促炎细胞因子（TNF、IL-1 β、IFN-γ、IL-17）含量增加]。观察到引流（肠系膜）淋巴结（MLN）应激反应[MLN谷胱甘肽（GSH）和金属硫蛋白（MT）mRNA水平升高]和适应性刺激[细胞构成、增殖、促炎（IFN-γ和IL-17）MLN细胞因子反应]以及先天性免疫活性（NK和CD 68（+）细胞数量增加、氧化活性、IL-1 β）。与MLN中的促炎性环境相反，观察到IL-10应答降低或不变。MLN细胞的免疫活性的刺激，最有可能的是，由于镉诱导的组织损伤的传感器，但也从细菌抗原，打破了受损的肠屏障。在评估膳食镉作为健康风险因素时，应考虑镉的这些影响。(C)2015爱思唯尔爱尔兰有限公司版权所有。
Gastrointestinal tract is one of the main targets of cadmium (Cd), an important food and drinking water contaminant. In the present study, the effect of subchronic (30 days) oral (in water) intake of 5ppm and 50ppm of cadmium on immune responses in the gut was examined in rats. Cadmium consumption resulted in reduction of bacteria corresponding to Lactobacillus strain, tissue damage and intestinal inflammation [increases in high mobility group box 1 (HMGB1 molecules), superoxide dismutase (SOD) and catalase (CAT) activity and proinflammatory cytokine (TNF, IL-1 beta, IFN-gamma, IL-17) content]. Draining (mesenteric) lymph node (MLN) stress response was observed [elevation of MLN glutathione (GSH) and metallothionein (MT) mRNA levels] and stimulation of both adaptive [cellularity, proliferation, proinflammatory (IFN-gamma and IL-17) MLN cell cytokine responses] as well as innate immune activity (increases in numbers of NK and CD68(+) cells, oxidative activities, IL-1 beta). In contrast to proinflammatory milieu in MLN, decreased or unchanged antiinflammatory IL-10 response was observed. Stimulation of immune activities of MLN cells have, most probably, resulted from sensing of cadmium-induced tissue injury, but also from bacterial antigens that breached compromised intestinal barrier. These effects of cadmium should be taken into account when assessing dietary cadmium as health risk factor. (C) 2015 Elsevier Ireland Ltd. All rights reserved.