Endothelial and hematopoietic cell fate of human embryonic stem cells originates from primitive endothelium with hemangioblastic properties
Endothelial and hematopoietic cell fate of human embryonic stem cells originates from primitive endothelium with hemangioblastic properties
复制标题
DOI：
10.1016/j.immuni.2004.06.006
复制
发表时间：
2004-07-01
期刊：
影响因子：
32.4
通讯作者：
Bhatia, M
中科院分区：
文献类型：
--
作者：
Wang, LS;Li, L;Bhatia, M
在人类中启动胚胎血管生成和造血的前体之间的细胞组织和关系尚未被表征。在这里，我们确定了一个亚群的原始内皮样细胞来源于人胚胎干细胞（hESC），表达PECAM-1，Flk-1，和VE-钙粘蛋白，但不CD 45（CD 45（阴性）PFV细胞），是唯一负责内皮和造血发育。CD 45（阴性）PFV细胞的分子特征与内皮和造血能力一致。克隆分离表明，CD 45（阴性）PFV群体包括具有内皮和造血能力的双能细胞。我们认为，人类造血和内皮细胞的成熟完全起源于一个子集的胚胎内皮细胞，具有成血管细胞的特性，并提供了一个模型系统，以研究这些谱系关系的人。
The cellular organization and relationships among precursors that initiate embryonic angiogenesis and hematopoiesis in the human have yet to be characterized. Here, we identify a subpopulation of primitive endothelial-like cells derived from human embryonic stem cells (hESCs) that express PECAM-1, Flk-1, and VE-cadherin, but not CD45 (CD45(neg)PFV cells), and that are uniquely responsible for endothelial and hematopoietic development. Molecular profiling of CD45(neg)PFV cells is consistent with endothelial and hematopoietic competency. Clonal isolation demonstrates that the CD45(neg)PFV population includes bipotent cells with endothelial and hematopoietic capacity. We suggest that human hematopoiesis and endothelial maturation originate exclusively from a subset of embryonic endothelium that possesses hemangioblastic properties and offers a model system to study these lineage relationships in the human.