New virulence-activated and virulence-repressed genes identified by systematic gene inactivation and generation of transcriptional fusions in Bordetella pertussis
New virulence-activated and virulence-repressed genes identified by systematic gene inactivation and generation of transcriptional fusions in Bordetella pertussis
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DOI：
10.1128/jb.182.20.5902-5905.2000
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发表时间：
2000-10-01
影响因子：
3.2
通讯作者：
Jacob-Dubuisson, F
中科院分区：
文献类型：
--
作者：
Antoine, R;Alonso, S;Jacob-Dubuisson, F
对百日咳杆菌部分完成的基因组序列进行了电子扫描，并分析了与新的整合载体产生的转录融合，以确定新的潜在毒力基因。编码铁载体受体、粘附素和自身转运蛋白的基因似乎受到全球毒力调节因子BvgAS的类似于波氏杆菌毒力基因的调控。相反，编码一种假定的内质素样蛋白的基因似乎在毒力条件下受到抑制。
An in silico scan of the partially completed genome sequence of Bordetella pertussis and analyses of transcriptional fusions generated with a new integrational vector were used to identify new potential virulence genes. The genes encoding a putative siderophore receptor, adhesins, and an autotransporter protein appeared to be regulated in a manner similar to Bordetella virulence genes by the global virulence regulator BvgAS. In contrast, the gene encoding a putative intimin-like protein appeared to be repressed under conditions of virulence.