Benzothiadiazole-induced priming for potentiated responses to pathogen infection, wounding, and infiltration of water into leaves requires the NPR1/NIM1 gene in Arabidopsis
Benzothiadiazole-induced priming for potentiated responses to pathogen infection, wounding, and infiltration of water into leaves requires the NPR1/NIM1 gene in Arabidopsis
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DOI：
10.1104/pp.010744
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发表时间：
2002-03-01
期刊：
影响因子：
7.4
通讯作者：
Conrath, U
中科院分区：
文献类型：
--
作者：
Kohler, A;Schwindling, S;Conrath, U
系统获得性抗性(SAR)是一种植物的防御状态，例如，在以前的病原体感染后或被模拟天然信号化合物的化学物质诱导后。与未诱导的植物相比，合成孔径雷达与更快、更大程度地诱导细胞防御反应的能力有关，这一过程被称为“引发”。用合成的SAR诱导剂苯并噻二唑(BTH)处理拟南芥植株，然后再刺激两个显著的细胞防御反应，即Phe解氨酶(PAL)基因的激活和愈伤组织的沉积，尽管BTH本身在诱导这两个反应时基本上是无效的，但BTH预处理后大大增强了随后由紫丁香假单胞菌诱导的PAL基因的表达。番茄侵染、伤害或渗入叶片的病原菌。BTH处理还能促进因水分伤害或渗入叶片而产生的类胡萝卜素的产生。有趣的是，在拟南芥PR基因1/非诱导免疫1突变体中未发现BTH对PAL基因的激活和痂蛋白沉积的增强作用，而这一突变体在SAR中受损。相反，在病程相关基因(CPR)1的组成型表达株和病程相关基因5的组成型表达突变体中，在没有BTH处理的情况下，PAL基因的激活和愈伤组织的合成都得到了增强。此外，在病原菌诱导的SAR中也发现了增强防御基因激活的启动。综上所述，这些结果表明，启动是植物获得抗病的一个重要的细胞机制，它需要PR基因1/非标记免疫1基因的非表达。
Systemic acquired resistance (SAR) is a plant defense state that is induced, for example, after previous pathogen infection or by chemicals that mimic natural signaling compounds. SAR is associated with the ability to induce cellular defense responses more rapidly and to a greater degree than in noninduced plants, a process called "priming." Arabidopsis plants were treated with the synthetic SAR inducer benzothiadiazole (BTH) before stimulating two prominent cellular defense responses, namely Phe AMMONIA-LYASE (PAL) gene activation and callose deposition, Although BTH itself was essentially inactive at the immediate induction of these two responses, the pretreatment with BTH greatly augmented the subsequent PAL gene expression induced by Pseudomonas syringae pv. tomato infection, wounding, or infiltrating the leaves with v,,ater. The BTH pretreatment also enhanced the production of callose, which was induced by wounding or infiltrating the leaves with water. It is interesting that the potentiation by BTH pretreatment of PAL gene activation and callose deposition was not seen in the Arabidopsis nonexpresser of PR genes 1/noninducible immunity 1 mutant, which is compromised in SAR. In a converse manner, augmented PAL gene activation and enhanced callose biosynthesis were found, without BTH pretreatment, in the Arabidopsis constitutive expresser of pathogenesis-related genes (cpr)1 and constitutive expresser of pathogenesis-related genes 5 mutants, in which SAR is constitutive. Moreover, priming for potentiated defense gene activation was also found in pathogen-induced SAR. In sum, the results suggest that priming is an important cellular mechanism in acquired disease resistance of plants that requires the nonexpresser of PR genes 1/nonindticible immunity 1 gene.