Weissella oryzae sp. nov., isolated from fermented rice grains.
Weissella oryzae sp. nov., isolated from fermented rice grains.
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DOI：
10.1099/ijs.0.043612-0
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发表时间：
2013-04
影响因子：
2.8
通讯作者：
M. Tohno;M. Kitahara;Hidehiko Inoue;R. Uegaki;T. Irisawa;M. Ohkuma;K. Tajima
中科院分区：
文献类型：
--
作者：
M. Tohno;M. Kitahara;Hidehiko Inoue;R. Uegaki;T. Irisawa;M. Ohkuma;K. Tajima
对从日本发酵米中分离到的两株革兰氏反应阳性、过氧化氢酶阴性、不规则短杆状或球形乳酸菌（命名为SG 25（T）和SG 23）进行了分类研究。亚种japonica）。基于16 S rRNA基因序列数据的系统发育分析清楚地表明，这些菌株属于Weissella属，并且与Weissella soli LMG 20113（T）关系最密切（每个新菌株的序列相似性为96.9%）。每种菌株的肽聚糖分别含有摩尔比为1.0：1.2：0.5：3.0的氨基酸谷氨酸、赖氨酸、丝氨酸和丙氨酸。基于新菌株的不寻常的表型特征和每个新菌株与Weissella soli JCM 12536（T）之间记录的低水平的DNA-DNA相关性，菌株SG 25（T）和SG 23代表Weissella属中的单个新物种，提议将其命名为Weissella sp. nov.。模式菌株是SG 25（T）（= JCM 18191（T）= DSM 25784（T））。
A taxonomic study was conducted on two Gram-reaction-positive, catalase-negative, irregular short-rod-shaped or coccoid lactic acid bacteria, designated strains SG25(T) and SG23, that were isolated from grains of fermented Japanese rice (Oryza sativa L. subsp. japonica). A phylogenetic analysis based on 16S rRNA gene sequence data clearly showed that the strains belonged to the genus Weissella and were most closely related to Weissella soli LMG 20113(T) (with a sequence similarity of 96.9 % for each novel strain). The peptidoglycan of each strain contained the amino acids glutamic acid, lysine, serine and alanine in a molar ratio of 1.0 : 1.2 : 0.5 : 3.0, respectively. On the basis of the unusual phenotypic characteristics of the novel strains and the low levels of DNA-DNA relatedness recorded between each novel strain and Weissella soli JCM 12536(T), strains SG25(T) and SG23 represent a single novel species in the genus Weissella, for which the name Weissella oryzae sp. nov. is proposed. The type strain is SG25(T) ( = JCM 18191(T) = DSM 25784(T)).