Chloride Intracellular Channel 5 Modulates Adipocyte Accumulation in Skeletal Muscle by Inhibiting Preadipocyte Differentiation
Chloride Intracellular Channel 5 Modulates Adipocyte Accumulation in Skeletal Muscle by Inhibiting Preadipocyte Differentiation
复制标题
氯离子细胞内通道 5 通过抑制前脂肪细胞分化来调节骨骼肌中脂肪细胞的积累
DOI：
10.1002/jcb.22615
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发表时间：
2010-07-01
影响因子：
4
通讯作者：
Du, Min
中科院分区：
文献类型：
--
作者：
Li, Feng-Na;Yin, Jing-Dong;Du, Min
肌内脂肪，骨骼肌内沉积的总脂质，被认为是影响动物肉质和人类胰岛素抵抗的潜在因素。本研究的目的是通过动物模型确定控制肌肉内脂肪积累的候选基因。选用PIC猪（瘦型）和荣昌猪（肥胖型）。利用Affymetrix基因芯片芯片技术对背最长肌mRNA样本进行扫描，分离出了猪背最长肌细胞内氯化sus scrofa channel 5 (CLIC5)，其mRNA丰度和蛋白表达水平与肌内脂肪含量呈负相关。此外，过表达CLIC5显著增加3T3-L1前脂肪细胞的增殖，抑制脂肪细胞分化，同时下调c/EBP α、LPL和PPAR γ蛋白。我们的研究结果表明，CLIC5可能是猪骨骼肌脂肪积累的关键调节剂。j .细胞。中国生物工程学报，2010,31(2):393 - 398。(C) 2010 Wiley-Liss。公司。
Intramuscular fat, the total lipid deposited within skeletal muscle, has been regarded as a potential factor responsible for meat quality in animal production and insulin resistance in humans. The objective of present study was to identify candidate genes which control intramuscular fat accumulation through using animal models. PIC pigs (lean-type) and Rongchang pigs (obese-type) were used. By scanning the mRNA samples of longissimus dorsi muscle with Affymetrix Gene-Chip microarray technology, sus scrofa chloride intracellular channel 5 (CLIC5) was isolated, and its mRNA abundance and protein expression level were reversely related with the intramuscular fat content of pigs. Furthermore, over-expression of CLIC5 dramatically increased the proliferation of 3T3-L1 preadipocytes, while inhibited adipocytic differentiation accompanied by the down-regulation of c/EBP alpha, LPL, and PPAR gamma protein. Our results suggest that CLIC5 might be a crucial regulator of adipose accumulation in skeletal muscle of pigs. J. Cell. Biochem. 110: 1013-1021, 2010. (C) 2010 Wiley-Liss. Inc.