Prognostic value of tumor-infiltrating Foxp3+T-cell subpopulations in metastatic melanoma
Prognostic value of tumor-infiltrating Foxp3+T-cell subpopulations in metastatic melanoma
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DOI：
10.1111/j.1600-0625.2011.01260.x
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发表时间：
2011-05-01
影响因子：
3.6
通讯作者：
Dreno, Brigitte
中科院分区：
文献类型：
--
作者：
Knol, Anne C.;Nguyen, Jean M.;Dreno, Brigitte
调节性T细胞已经与各种类型癌症的不良预后有关。此前有报道称，在卵巢癌中，Foxp3的定量鉴定出一组预后明显较差的患者（总生存期（OS）和无进展生存期（PFS）），这表明Foxp3的高表达水平可能是促进肿瘤免疫逃逸的免疫抑制微环境的替代标志物。本研究的主要目的是明确Foxp3对III期（AJCC）黑色素瘤患者PFS和OS的预后价值。从102个转移性黑色素瘤淋巴结和8个无瘤淋巴结中分离总RNA。对Foxp3进行实时荧光定量PCR检测，并与患者预后相关。Foxp3的量化确定了一个患者亚组（bbb90百分位），其特征是PFS预后明显较差（P = 0.000271），但OS预后较差（P = 0.11）。总之，使用qPCR定量Foxp3表达似乎是III期黑色素瘤患者（AJCC） PFS的独立预后因素。因此，Foxp3的高表达可能有助于识别复发风险最高的患者。
Regulatory T cells have already been associated with poor prognosis in various types of cancer. It was previously reported, in ovarian carcinoma, that quantification of Foxp3 identified a subgroup of patients characterized by a significantly worse prognosis in terms of overall survival (OS) and progression-free survival (PFS), suggesting that high expression levels of Foxp3 might represent a surrogate marker for an immunosuppressive microenvironment contributing to tumor immune escape. The main objective of the present study was to precise the prognostic value of Foxp3 regarding PFS and OS in stage III (AJCC) melanoma patients. Total RNA was isolated from 102 metastatic melanoma lymph nodes and from eight tumor-free lymph nodes. Real-time PCR for Foxp3 was performed and correlated with patients' outcome. Quantification of Foxp3 identified a patient subgroup (> 90th percentile), which is characterized by a significantly worse prognosis in terms of PFS (P = 0.000271) but not in terms of OS (P = 0.11).In conclusion, quantification of Foxp3 expression using qPCR appears as an independent prognostic factor for PFS in stage III melanoma patients (AJCC). High Foxp3 expression might thus enable the identification of patients most at risk of relapse.