Different Effects of Aluminum on the Actin Cytoskeleton and Brefeldin A-Sensitive Vesicle Recycling in Root Apex Cells of Two Maize Varieties Differing in Root Elongation Rate and Aluminum Tolerance
Different Effects of Aluminum on the Actin Cytoskeleton and Brefeldin A-Sensitive Vesicle Recycling in Root Apex Cells of Two Maize Varieties Differing in Root Elongation Rate and Aluminum Tolerance
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DOI：
10.1093/pcp/pcp013
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发表时间：
2009-03-01
影响因子：
4.9
通讯作者：
Barcelo, Juan
中科院分区：
文献类型：
--
作者：
Amenos, Montse;Corrales, Isabel;Barcelo, Juan
铝毒、内吞作用、内体和囊泡循环在根过渡区的关系最近被证实。在这里，丝状肌动蛋白（F-肌动蛋白）为基础的小泡运输铝耐性的重要性已调查不同的玉米品种在其铝敏感性。铝敏感品种1636的根尖细胞比耐铝品种Cateto吸收更多的铝。肌动蛋白细胞骨架和囊泡运输是敏感品种根尖铝毒的主要目标。可视化硼交联的鼠李糖半乳糖醛酸聚糖II（RGII）含有布雷菲德菌素A（BFA）的车厢显示，铝抑制这些车厢的形成，特别是在品种1636。铝对果胶循环影响的时间顺序与铝对该敏感品种的生长影响相匹配。这些结果支持这一假设，铝结合到富含果胶的细胞壁可以有助于可逆抑制根伸长。铝诱导的F-肌动蛋白的变化是最明显的铝敏感的1636，其中铝定位在核仁内的过渡区的中心部分。关于这一观察结果，共质体铝在不可逆的生长抑制和改善BFA诱导的抑制根伸长的作用进行了讨论。
A relationship between aluminum (Al) toxicity, endocytosis, endosomes and vesicle recycling in the root transition zone has recently been demonstrated. Here the importance of filamentous actin (F-actin)-based vesicle trafficking for Al tolerance has been investigating in maize varieties differing in their Al sensitivities. More Al was internalized into root tip cells of the Al-sensitive variety 1636 than in the Al-tolerant variety Cateto. The actin cytoskeleton and vesicle trafficking were primary targets for Al toxicity in the root tips of the sensitive variety. Visualization of boron-cross-linked rhamnogalacturonan II (RGII)-containing brefeldin A (BFA) compartments revealed that Al inhibited the formation of these compartments, especially in variety 1636. The time sequence of Al effects on pectin recycling matches the growth effects of Al in this sensitive variety. These results support the hypothesis that Al binding to pectin-rich cell walls can contribute to reversible inhibition of root elongation. Al-induced alterations on F-actin were most evident in the central part of the transition zone of Al-sensitive 1636, where Al was localized inside the nucleoli. In relation to this observation, a role for symplastic Al in both irreversible growth inhibition and amelioration of BFA-induced inhibition of root elongation is discussed.