Expression and enzymatic activity of MMP-2 during healing process of the acute supraspinatus tendon tear in rabbits
Expression and enzymatic activity of MMP-2 during healing process of the acute supraspinatus tendon tear in rabbits
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DOI：
10.1016/s0736-0266(02)00016-5
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发表时间：
2002-09-01
影响因子：
2.8
通讯作者：
Iwata, H
中科院分区：
文献类型：
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作者：
Choi, HR;Kondo, S;Iwata, H
我们通过观察基质金属蛋白酶-2 （MMP-2）的表达及其随组织金属蛋白酶抑制剂（TIMPs）表达的酶活性随时间的变化，研究了兔手术所致的棘上肌腱撕裂的自然愈合过程。一个横向的，全层撕裂的冈上肌腱被创建和检查。免疫组化分析显示，MMP-2阳性细胞主要分布于肌腱切割两端，修复组织从法氏囊侧侵入间隙。在修复过程中，TIMP-1不仅在肌腱边缘的细胞中表达，而且在修复组织中也被诱导表达。TIMP-2在肌腱和修复组织中均有组成性表达。使用组织培养基的明胶酶谱图显示了MMP-2的潜伏和活性形式以及酶活性的特征时间链变化。Western blotting证实这些条带为MMP-2的潜伏形式。这些结果表明，MMP-2在急性冈上肌腱撕裂愈合过程中表达和激活，并在重塑过程中发挥重要作用。(C) 2002骨科研究学会。Elsevier Science Ltd.出版。版权所有。
We investigated the spontaneous healing process of a Surgically created supraspinatus tendon tear in rabbits with specific reference to the expression of matrix metalloproteinase-2 (MMP-2) and its time-course change in enzymatic activity along with the expression of tissue inhibitors of metalloproteinases (TIMPs). A transverse, full thickness tear of the supraspinatus tendon was created and examined. Immunohistochemical analysis revealed that MMP-2 positive cells were mainly localized at both cutting ends of the tendon, and reparative tissue encroached into the gap from the bursal side. The expression of TIMP-1 was induced in the cells at not only the tendon edges but also the reparative tissue during the healing process. TIMP-2 was Constitutively expressed in both the tendon and the reparative tissue. Gelatin zymography using tissue culture media demonstrated latent and active forms of MMP-2 and characteristic time-linked changes of the enzymatic activity. Western blotting confirmed the bands as the latent form of MMP-2. These results suggest that MMP-2 is expressed and activated during the healing process of acute supraspinatus tendon tear and can play an important role in the remodeling process. (C) 2002 Orthopaedic Research Society. Published by Elsevier Science Ltd. All rights reserved.