Topographic, electrochemical, and optical images captured using standing approach mode scanning electrochemical/optical microscopy.
Topographic, electrochemical, and optical images captured using standing approach mode scanning electrochemical/optical microscopy.
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DOI：
10.1021/la0611763
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发表时间：
2006-08
期刊：
影响因子：
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通讯作者：
Yasufumi Takahashi;Y. Hirano;T. Yasukawa;H. Shiku;H. Yamada;T. Matsue
中科院分区：
文献类型：
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作者：
Yasufumi Takahashi;Y. Hirano;T. Yasukawa;H. Shiku;H. Yamada;T. Matsue
我们开发了一种高分辨率扫描电化学显微镜（SECM），用于各种生物材料的表征。电极探针通过Ti/Pt溅射，然后通过聚对二甲苯C-气相沉积聚合在拉动的光纤或玻璃毛细管上制备。从亚铁氰化物的循环伏安图估计的有效电极半径被发现是35 nm。光学孔径尺寸小于170 nm，这是从直径在10-15 nm范围内的量子点（QD）颗粒的近场扫描光学显微镜（NSOM）图像的横截面证实的。改进了控制探针-样品距离的反馈机制，使探针垂直移动0.1-3微米，以减少对样品的损伤。这种反馈模式被定义为“站立接近（STA）模式”（Yamada，H.; Fukumoto，H.; Yokoyama，T.;小池，T. Anal. 2005，77，1785-1790）首次允许在生理条件下对单个PC 12细胞的轴突和细胞体同时进行电化学和拓扑成像。STA模式的反馈成像功能比传统的AFM针尖样品调节更好。例如，聚苯乙烯珠（直径约6微米）使用STA模式SECM成像，而使用常规AFM仪器不可能成像。
We developed a high-resolution scanning electrochemical microscope (SECM) for the characterization of various biological materials. Electrode probes were fabricated by Ti/Pt sputtering followed by parylene C-vapor deposition polymerization on the pulled optical fiber or glass capillary. The effective electrode radius estimated from the cyclic voltammogram of ferrocyanide was found to be 35 nm. The optical aperture size was less than 170 nm, which was confirmed from the cross section of the near-field scanning optical microscope (NSOM) image of the quantum dot (QD) particles with diameters in the range of 10-15 nm. The feedback mechanism controlling the probe-sample distance was improved by vertically moving the probe by 0.1-3 microm to reduce the damage to the samples. This feedback mode, defined as "standing approach (STA) mode" (Yamada, H.; Fukumoto, H.; Yokoyama, T.; Koike, T. Anal. Chem. 2005, 77, 1785-1790), has allowed the simultaneous electrochemical and topographic imaging of the axons and cell body of a single PC12 cell under physiological conditions for the first time. STA-mode feedback imaging functions better than tip-sample regulation by the conventionally available AFM. For example, polystyrene beads (diameter approximately 6 microm) was imaged using the STA-mode SECM, whereas imaging was not possible using a conventional AFM instrument.