An injectable robust denatured albumin hydrogel formed via double equilibrium reactions
An injectable robust denatured albumin hydrogel formed via double equilibrium reactions
复制标题
通过双平衡反应形成可注射的坚固变性白蛋白水凝胶
DOI：
10.1080/09205063.2019.1600821
复制
发表时间：
2019-05-24
影响因子：
3.6
通讯作者：
Yuan, Xubo
中科院分区：
文献类型：
--
作者：
Bai, Yu;Li, Sidi;Yuan, Xubo
摘要蛋白质是制备生物材料的理想原料，具有比合成高分子更好的生物相容性和更大的弹性范围。可注射水凝胶对于微创策略是高度期望的。目前，利用天然材料制备综合性能良好的可注射水凝胶越来越受到人们的重视。本文采用尿素和谷胱甘肽处理牛血清白蛋白（BSA），制备了由两个动态平衡键（二硫键和氢键）控制的BSA水凝胶。尿素-蛋白质、蛋白质-蛋白质之间的氢键平衡以及巯基-二硫键之间的交换反应平衡不仅有利于提高体系的力学性能，而且使凝胶化时间缩短至5 min。此外，这种新型的白蛋白水凝胶被证明是良好的生物相容性。因此，本文所制备的白蛋白水凝胶在生理范围内可注射，细胞毒性低，力学性能好（储能模量可达10 kPa以上，压应力可达0.2 MPa），注射性好，具有良好的前景。
Abstract Proteins are ideal raw materials for preparing biomaterials with better biocompatibility and larger elastic range than synthetic polymers. Injectable hydrogels are highly desired for minimal invasive strategies. At present, preparation of injectable hydrogels with good comprehensive properties using natural materials receives more and more attention. In this article, BSA was treated with urea and glutathione to prepare bovine serum albumin hydrogel controlled by two dynamic equilibrium bonds (disulfide and hydrogen bonds). The hydrogen bond equilibrium between urea-protein and protein-protein as well as the exchange reaction equilibrium between thiol-disulfide bonds in the system not only facilitated to enhance the mechanical properties, but also to reduce the gelation time to 5 min. Moreover, this novel albumin hydrogel was proved to be of good biocompatibility. Therefore, the albumin hydrogels in this article were injectable within the physiological range and shown low cytotoxicity, good machanical property ( storage modulus could be greater than 10 kPa and compressive stress could reach 0.2 MPa) and good injectability, which exhibited promising prospect.