MALDI-TOF Mass Spectrometry Discriminates Known Species and Marine Environmental Isolates of Pseudoalteromonas.
MALDI-TOF Mass Spectrometry Discriminates Known Species and Marine Environmental Isolates of Pseudoalteromonas.
复制标题
DOI：
10.3389/fmicb.2016.00104
复制
发表时间：
2016
影响因子：
5.2
通讯作者：
Mesbahi E
中科院分区：
文献类型：
--
作者：
Emami K;Nelson A;Hack E;Zhang J;Green DH;Caldwell GS;Mesbahi E
假交替单胞菌属是一类具有重要生态意义的海洋γ-变形菌，作为生物活性化合物和酶的生产者具有潜在的生物技术价值。了解它们在环境中的作用和新产品的生物勘探取决于识别环境分离物的有效方法。基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF MS）生物分型有望成为鉴定和区分不同类型细菌的快速可靠的方法，但对海洋细菌的应用相对有限，并且尚未系统地应用于假交替单胞菌。因此，我们构建了一个MALDI-TOF MS数据库的31个已知的假交替单胞菌物种，新的菌株可以比较MALDI-TOF生物分型。通过与16 S rRNA基因测序进行比较，仔细检查了MALDI-TOF MS区分物种的能力。这两种方法给出的相似性模式大致一致，但并不完全一致。总体而言，MALDI-TOF MS的分辨率高于16 S rRNA基因测序。该数据库进行了测试与13个环境假交替单胞菌分离物从英国沃茨。所有供试菌株的MALDI-TOF MS生物分型均能在属水平上进行鉴定，但大多数菌株不能在种水平上进行明确鉴定。我们的结论是，这些分离株中的几个，可能是大多数，代表新的物种。因此，在MALDI-TOF MS生物分型可以可靠地用于物种鉴定之前，需要对假交替单胞菌进行进一步的分类学研究。然而，它是一个强大的工具，用于表征和区分环境菌株，并可以作出重要贡献的分类研究。
The genus Pseudoalteromonas constitutes an ecologically significant group of marine Gammaproteobacteria with potential biotechnological value as producers of bioactive compounds and of enzymes. Understanding their roles in the environment and bioprospecting for novel products depend on efficient ways of identifying environmental isolates. Matrix Assisted Laser Desorption/Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS) biotyping has promise as a rapid and reliable method of identifying and distinguishing between different types of bacteria, but has had relatively limited application to marine bacteria and has not been applied systematically to Pseudoalteromonas. Therefore, we constructed a MALDI-TOF MS database of 31 known Pseudoalteromonas species, to which new isolates can be compared by MALDI-TOF biotyping. The ability of MALDI-TOF MS to distinguish between species was scrutinized by comparison with 16S rRNA gene sequencing. The patterns of similarity given by the two approaches were broadly but not completely consistent. In general, the resolution of MALDI-TOF MS was greater than that of 16S rRNA gene sequencing. The database was tested with 13 environmental Pseudoalteromonas isolates from UK waters. All of the test strains could be identified to genus level by MALDI-TOF MS biotyping, but most could not be definitely identified to species level. We conclude that several of these isolates, and possibly most, represent new species. Thus, further taxonomic investigation of Pseudoalteromonas is needed before MALDI-TOF MS biotyping can be used reliably for species identification. It is, however, a powerful tool for characterizing and distinguishing among environmental isolates and can make an important contribution to taxonomic studies.