Uncoupling of brain activity from movement defines arousal states in Drosophila
Uncoupling of brain activity from movement defines arousal states in Drosophila
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DOI：
10.1016/j.cub.2003.12.057
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发表时间：
2004-01-20
期刊：
影响因子：
9.2
通讯作者：
Greenspan, RJ
中科院分区：
文献类型：
--
作者：
van Swinderen, B;Nitz, DA;Greenspan, RJ
背景：动物的觉醒状态是其所有行为的基础。唤醒通常通过运动测量来确定，并辅之以大脑活动记录，其可以提供独立于运动活动的签名。在这里，我们研究运动，觉醒状态，和局部场电位（LFP）活动之间的关系在果蝇brain.Results：我们已经测量了局部场电位（LFP）在大脑和果蝇在不同状态的觉醒，如自发白天清醒运动，视觉觉醒，自发夜间运动，刺激诱导的运动的明显运动之间的相关性。我们发现，脑LFP活动和运动之间的相关强度依赖于行为状态，并在一定程度上，对LFP频率范围。在视觉刺激的呈现过程中，以及在黑暗中过夜的实验过程中，大脑活动和运动是分离的。时代的低相关性或解耦的预测增加的唤醒阈值，即使在移动的苍蝇，从而定义一个独特的状态之间的唤醒中间的睡眠和清醒的果蝇。结论：这些实验表明，大脑LFPs和运动之间的关系在果蝇是动态的，这两个活动的措施之间的耦合程度定义不同的状态的唤醒。
Background: An animal's state of arousal is fundamental to all of its behavior. Arousal is generally ascertained by measures of movement complemented by brain activity recordings, which can provide signatures independently of movement activity. Here we examine the relationships among movement, arousal state, and local field potential (LFP) activity in the Drosophila brain.Results: We have measured the correlation between local field potentials (LFPs) in the brain and overt movements of the fruit fly during different states of arousal, such as spontaneous daytime waking movement, visual arousal, spontaneous night-time movement, and stimulus-induced movement. We found that the correlation strength between brain LFP activity and movement was dependent on behavioral state and, to some extent, on LFP frequency range. Brain activity and movement were uncoupled during the presentation of visual stimuli and also in the course of overnight experiments in the dark. Epochs of low correlation or uncoupling were predictive of increased arousal thresholds even in moving flies and thus define a distinct state of arousal intermediate between sleep and waking in the fruit fly.Conclusions: These experiments indicate that the relationship between brain LFPs and movement in the fruit fly is dynamic and that the degree of coupling between these two measures of activity defines distinct states of arousal.