Modulation of Cl-, K+, and nonselective cation conductances by taurine in olfactory receptor neurons of the mudpuppy Necturus maculosus.
Modulation of Cl-, K+, and nonselective cation conductances by taurine in olfactory receptor neurons of the mudpuppy Necturus maculosus.
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DOI：
10.1085/jgp.101.4.469
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发表时间：
1993-04
期刊：
影响因子：
--
通讯作者：
Dionne VE
中科院分区：
文献类型：
--
作者：
Dubin AE;Dionne VE
气味是通过调节嗅觉受体神经元膜电导的过程来转导的。在不使用酶的情况下，对水生蝾螈 Necturus maculosus 的嗅觉神经元进行了精密分离，并使用电阻全细胞方法进行研究，以尽量减少可溶性细胞内成分的损失。 224 个神经元中有 55 个对浓度在 10 nM 至 100 µM 之间的测试化合物牛磺酸有反应。牛磺酸引起四种不同的电导变化：Cl-电导增加（33%）、非选择性阳离子电导增加（15%）、Cl-电导降低（15%）和K+电导降低（15%）；此外，一些神经元引起的反应太小而无法表征。在大多数情况下，牛磺酸似乎只调节任何特定细胞中的单一电导。每个电导的调节都是剂量依赖性的，并且每个反应在正常全细胞模式下快速下降，这可能是由于转导途径中可扩散成分的冲刷所致。牛磺酸敏感电导的调节引起抑制性或兴奋性反应。体内类似的反应多样性会产生复杂的电活动模式，可以编码气味的特性和特征。
Odors are transduced by processes that modulate the membrane conductance of olfactory receptor neurons. Olfactory neurons from the aquatic salamander, Necturus maculosus, were acutely isolated without enzymes and studied with a resistive whole-cell method to minimize loss of soluble intracellular constituents. 55 of 224 neurons responded to the test compound taurine at concentrations between 10 nM and 100 microM. Four different conductance changes were elicited by taurine: an increased Cl- conductance (33%), an increased nonselective cation conductance (15%), a decreased Cl- conductance (15%), and a decreased K+ conductance (15%); in addition, responses too small to be characterized were elicited in some neurons. In most cases, taurine appeared to modulate only a single conductance in any particular cell. Modulation of each conductance was dose dependent, and each response ran down quickly in the normal whole-cell mode, presumably due to washout of a diffusible component in the transduction pathway. Modulation of taurine-sensitive conductances caused either inhibitory or excitatory responses. A similar diversity of responses in vivo would produce a complex pattern of electrical activity that could encode the identity and characteristics of an odor.